Miroshnikov A I
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
Biofizika. 1998 Nov-Dec;43(6):1032-6.
The relationship between the inhibitory effect of sodium chloride and potassium anolites, obtained in a diaphragm electrolyser, and the physicochemical parameters of solutions was compared with that between the inhibitory effect and physicochemical properties of hypochlorites obtained after treating the solutions in an electrolyser having no diaphragm was compared. The biological activity of solutions containing molecular chlorine, hypochlorous acid, and hypochlorite ions was determined by their effect on the growth of E. coli cells. After a 5-min incubation of cells with each of the oxidizers, the bacterial growth stopped and was not restored during one day. The conclusion is made that the oxidizers irreversibly disturb the barrier properties of cell membranes and, in some cases, destroy cells. In model solutions, as well as in solutions treated after heating on a water bath or after the addition of sodium thiosulfate, a delay in the start of E. coli growth occurs. After the lag-phase, the repair of cells sets on, and after a day the optical density of cells increases and approaches the control.
比较了在隔膜电解槽中获得的氯化钠和钾沸石的抑制作用与溶液的物理化学参数之间的关系，以及在无隔膜电解槽中处理溶液后获得的次氯酸盐的抑制作用与物理化学性质之间的关系。通过含分子氯、次氯酸和次氯酸根离子的溶液对大肠杆菌细胞生长的影响来测定其生物活性。在用每种氧化剂将细胞孵育5分钟后，细菌生长停止且在一天内未恢复。得出的结论是，这些氧化剂会不可逆地扰乱细胞膜的屏障特性，在某些情况下还会破坏细胞。在模型溶液中，以及在水浴加热或添加硫代硫酸钠后处理的溶液中，大肠杆菌生长开始出现延迟。在延迟期之后，细胞开始修复，一天后细胞的光密度增加并接近对照值。
