Colazingari Sandra, Fiorenza Maria Teresa, Carlomagno Gianfranco, Najjar Robert, Bevilacqua Arturo
Department of Psychology, Section of Neuroscience, Sapienza University of Rome, via dei Marsi 78, 00185, Rome, Italy.
J Assist Reprod Genet. 2014 Apr;31(4):463-9. doi: 10.1007/s10815-014-0188-1. Epub 2014 Feb 14.
Myo-inositol (myoIns) has a positive role in mammalian development and human reproduction. Since experiments on farming species suggest a similar role in preimplantation development, we evaluated the hypothesis that the inclusion of myoIns in human embryo culture media would produce an increase in embryo quality in IVF cycles, using the mouse embryo assay.
To determine the effect of myoIns on completion of preimplantation development in vitro, one-cell embryos of the inbred C57BL/6N mouse strain were produced by ICSI, cultured in human fertilization media in the presence of myoIns (myoIns+) or in its absence (myoIns-) and evaluated morphologically. Daily progression through cleavage stages, blastocyst production and expansion and blastomere number at 96 hours post fertilization were assessed.
Compared to myoIns- embryos, myoIns+ embryos displayed a faster cleavage rate and by the end of preimplantation development, the majority of myoIns+ blastocysts was expanded and formed by a higher number of blastomeres.
The presence of myoIns resulted in both an increase in proliferation activity and developmental rate of in vitro cultured early mouse embryos, representing a substantial improvement of culture conditions. These data may identify myoIns as an important supplement for human embryo preimplantation culture.
肌醇（myoIns）在哺乳动物发育和人类生殖中具有积极作用。鉴于针对养殖物种的实验表明其在植入前发育中可能具有类似作用，我们采用小鼠胚胎试验评估了在人类胚胎培养基中添加肌醇会提高体外受精（IVF）周期中胚胎质量这一假设。
为确定肌醇对体外植入前发育完成情况的影响，通过胞浆内单精子注射（ICSI）制备近交系C57BL/6N小鼠品系的单细胞胚胎，将其在添加肌醇（myoIns+）或不添加肌醇（myoIns-）的人类受精培养基中培养，并进行形态学评估。评估受精后96小时内胚胎通过卵裂阶段的每日进展、囊胚产生和扩张情况以及卵裂球数量。
与myoIns-胚胎相比，myoIns+胚胎显示出更快的卵裂率，到植入前发育结束时，大多数myoIns+囊胚已扩张且由更多的卵裂球形成。
肌醇的存在导致体外培养的早期小鼠胚胎的增殖活性和发育速率均增加，这代表了培养条件的显著改善。这些数据可能表明肌醇是人类胚胎植入前培养的重要补充物。
