Kondo M, Tanoue C, Shingu M, Kajimura K, Miyaji T, Imura Y, Egawa S, Hokayama Y, Taniguchi A, Haraguchi Y
Department of Microbiology, Kurume University School of Medicine, Japan.
Kekkaku. 1992 Feb;67(2):97-105.
The effect of the culture filtrate Gemella haemolysans in enhancing mycobacterial growth has been previously demonstrated. In the present studies, an attempt was made to confirm whether the addition of the filtrate into the medium would be an effective method to promote the rapid detection of mycobacteria in sputum specimens of patients. One-hundred and one sputum specimens pretreated with NaOH were inoculated with various media (Dubos, Dubos-agar, 1% Ogawa, Kudo PD, and Middlebrook 7H9) supplemented with the culture filtrate of Gemella haemolysans grown in blood-BHI or blood-HEM at the dilutions of 1/32 or 1/64. Addition of the filtrate reduced the amount of time required to detect mycobacterial growth (Mycobacterium tuberculosis, M. avium complex, and M. kansassi) by an average of 60-70%. The media containing the filtrate formed significantly larger numbers of colonies compared with the control media, and those colonies inoculated developed more rapidly in size. The findings clearly indicated that the culture filtrate promotes the effective growth of mycobacteria which cannot or only slowly grows in ordinary media. Also indicated was that the addition of the culture filtrate of Gemella haemolysans into media provides as a useful tool to allow the rapid diagnosis of mycobacterial diseases.
溶血孪生球菌培养滤液促进分枝杆菌生长的作用先前已得到证实。在本研究中，试图确认将该滤液添加到培养基中是否是促进快速检测患者痰液标本中分枝杆菌的有效方法。将101份经氢氧化钠预处理的痰液标本接种于添加了溶血孪生球菌在血BHI或血HEM中培养的培养滤液的各种培养基（杜波斯培养基、杜波斯琼脂培养基、1%小川培养基、工藤PD培养基和Middlebrook 7H9培养基）中，滤液稀释度为1/32或1/64。添加滤液使检测分枝杆菌生长（结核分枝杆菌、鸟分枝杆菌复合群和堪萨斯分枝杆菌）所需的时间平均减少了60% - 70%。与对照培养基相比，含有滤液的培养基形成的菌落数量明显更多，接种的菌落在大小上生长得更快。这些发现清楚地表明，培养滤液促进了在普通培养基中不能生长或生长缓慢的分枝杆菌的有效生长。还表明，将溶血孪生球菌培养滤液添加到培养基中可作为一种有用的工具，用于快速诊断分枝杆菌疾病。
