Matsuo Masako
Faculty of Home Science, Gifu Women's University, 80 Taromaru, Gifu 501-2592, Japan.
J Nutr Sci Vitaminol (Tokyo). 2004 Aug;50(4):291-4. doi: 10.3177/jnsv.50.291.
In this paper, the saccharification process of okara fiber with pectinase, xylanase and cellulase was investigated as a preliminary attempt to effectively utilize okara fiber. The solubilization of okara fiber was raised in proportion to the number of enzymes used; that is, by a single enzyme, two enzymes and three enzymes, in that order. The saccharification of okara fiber was much more completed by a combination of pectinase, xylanase and cellulase than by using individual preparations. This multi-enzyme system liberated sugars equivalent to 80% of the original okara fiber by weight. Moreover the structure of okara fiber gradually disintegrated by hydrolytic reactions with pectinase, xylanase and cellulase in turns. These results suggest that the saccharification of okara fiber progresses by the cooperative action of pectinase, xylanase and cellulase.
本文对豆渣纤维用果胶酶、木聚糖酶和纤维素酶进行糖化过程进行了研究，作为有效利用豆渣纤维的初步尝试。豆渣纤维的溶解率随着所用酶的数量增加而提高，即依次使用单一酶、两种酶和三种酶时呈现这种规律。与单独使用酶制剂相比，果胶酶、木聚糖酶和纤维素酶组合使用时，豆渣纤维的糖化效果要好得多。这种多酶体系释放出的糖相当于原始豆渣纤维重量的80%。此外，豆渣纤维的结构通过依次与果胶酶、木聚糖酶和纤维素酶的水解反应而逐渐分解。这些结果表明，果胶酶、木聚糖酶和纤维素酶的协同作用促进了豆渣纤维的糖化过程。
