Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Research Center for Molecular Biology, Institutes of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, PR China.
Bioresour Technol. 2014 Mar;155:258-65. doi: 10.1016/j.biortech.2013.12.113. Epub 2014 Jan 4.
Sugarcane bagasse (SCB) resulting from different pretreatments was hydrolyzed by enzyme cocktails based on replacement of cellulase (Celluclast 1.5 L:Novozym 188=1FPU:4pNPGU) by xylanase or pectinase at different proportions. Lignin content of NaOH pretreated SCB and hemicellulose content of H2SO4 pretreated SCB were the lowest. NaOH pretreatment showed the best for monosaccharide production among the four pretreatments. Synergism was apparently observed between cellulase and xylanase for monosaccharide production from steam exploded SCB (SESB), NaOH, and H2O2 pretreated SCB. No synergism was observed between cellulase and pectinase for producing glucose. Additionally, no synergism was present when H2SO4 pretreated SCB was used. Replacement of 20% of the cellulase by xylanase enhanced the glucose yield by 6.6%, 8.8%, and 9.5% from SESB, NaOH, and H2O2 pretreated SCB, respectively. Degree of synergism between cellulase and xylanase had positive relationship with xylan content and was affected by hydrolysis time.
来自不同预处理的甘蔗渣（SCB）通过酶混合物水解，该酶混合物通过用木聚糖酶或果胶酶以不同比例替代纤维素酶（Celluclast 1.5 L：Novozym 188=1FPU：4pNPGU）。NaOH 预处理的 SCB 的木质素含量和 H2SO4 预处理的 SCB 的半纤维素含量最低。在这四种预处理方法中，NaOH 预处理对单糖的生成效果最好。在蒸汽爆破 SCB（SESB）、NaOH 和 H2O2 预处理的 SCB 中，纤维素酶和木聚糖酶之间明显表现出协同作用。纤维素酶和果胶酶在生产葡萄糖时没有协同作用。此外，当使用 H2SO4 预处理的 SCB 时，没有协同作用。用木聚糖酶替代 20%的纤维素酶，可分别使 SESB、NaOH 和 H2O2 预处理的 SCB 的葡萄糖产量提高 6.6%、8.8%和 9.5%。纤维素酶和木聚糖酶之间的协同作用程度与木聚糖含量呈正相关，并受水解时间的影响。
