Katayama Kenta, Sakurai Isamu, Wada Hajime
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
FEBS Lett. 2004 Nov 5;577(1-2):193-8. doi: 10.1016/j.febslet.2004.10.009.
Cardiolipin (CL) is an anionic phospholipid with a dimeric structure. In eukaryotes, it is primarily localized in the inner membranes of mitochondria. Although the biosynthetic pathway of CL is well known, the gene for CL synthase has not been identified in any higher organisms. In this study, the CLS gene for a CL synthase has been identified in a higher plant, Arabidopsis thaliana. We have shown that the CLS gene encodes a CL synthase by demonstrating its ability to catalyze the reaction of CL synthesis from CDP-diacylglycerol and phosphatidylglycerol, and that CLS is targeted into mitochondria. These findings demonstrate that CLS is a CL synthase located in mitochondria.
心磷脂（CL）是一种具有二聚体结构的阴离子磷脂。在真核生物中，它主要定位于线粒体内膜。尽管CL的生物合成途径已为人熟知，但在任何高等生物中尚未鉴定出CL合酶的基因。在本研究中，已在高等植物拟南芥中鉴定出CL合酶的CLS基因。我们通过证明其催化由CDP - 二酰甘油和磷脂酰甘油合成CL反应的能力，表明CLS基因编码一种CL合酶，并且CLS定位于线粒体。这些发现证明CLS是一种位于线粒体中的CL合酶。
