Bettio Daniela, Rizzi Nicoletta, Colombo Piergiuseppe, Bianchi Paolo, Gaetani Paolo
Laboratorio di Citogenetica e Genetica Medica, Istituto Clinico Humanitas, Milan, Italy.
Cancer Genet Cytogenet. 2004 Nov;155(1):79-81. doi: 10.1016/j.cancergencyto.2004.03.010.
A fresh specimen from an intracranial tumor, which was histopatologically classified as a synovial sarcoma, was investigated cytogenetically. The direct method failed to give any information due to a lack of mitotic cells after treatment with 70% acetic acid, whereas 6-day cultures showed a 45,XY,del(6q),-10 karyotype. The histologic evaluation was consistent with a synovial sarcoma, which is well characterized from a cytogenetic perspective and involves a t(X;18)(p11;q11) in more than 95% of cases. Reverse-transcription polymerase chain reaction analysis did not show the presence of the SYT-SSX chimeric gene.
对一个颅内肿瘤的新鲜标本进行了细胞遗传学研究，该肿瘤经组织病理学分类为滑膜肉瘤。由于用70%乙酸处理后缺乏有丝分裂细胞，直接法未能提供任何信息，而6天的培养显示出45,XY,del(6q),-10核型。组织学评估与滑膜肉瘤一致，滑膜肉瘤从细胞遗传学角度有很好的特征，超过95%的病例涉及t(X;18)(p11;q11)。逆转录聚合酶链反应分析未显示SYT-SSX嵌合基因的存在。
