Lai Ren, Takeuchi Hajime, Jonczy Jan, Rees Huw H, Turner Philip C
Cell Regulation and Signalling Division, School of Biological Sciences, University of Liverpool, Biosciences Building, Crown Street, Liverpool L69 7ZB, UK.
Gene. 2004 Nov 24;342(2):243-9. doi: 10.1016/j.gene.2004.07.012.
A novel thrombin inhibitor named Amblin was identified from the haemolymph of the ixodid (hard) tick, Amblyomma hebraeum, and the coding cDNA was isolated from a tick cDNA library. This cDNA codes for a preprotein of 166 amino acids, including a predicted signal peptide composed of 15 amino acids N-terminal to the mature Amblin. The 151-amino-acid mature Amblin contains 14 cysteines and two Kunitz-like domains. It displays high sequence similarity with a tissue factor pathway inhibitor (TFPI), Ixolaris, from the ixodid tick, Ixodes scapularis, which has 10 cysteines, and a thrombin inhibitor, Boophilin, from the ixodid tick, Boophilus microplus, which has 12 cysteines. Recombinant Amblin specifically inhibited thrombin as efficiently as native Amblin did. This is the first report of a thrombin inhibitor from tick haemolymph.
从硬蜱（璃眼蜱属）血红扇头蜱的血淋巴中鉴定出一种名为Amblin的新型凝血酶抑制剂，并从蜱cDNA文库中分离出其编码cDNA。该cDNA编码一个由166个氨基酸组成的前体蛋白，包括一个预测的信号肽，该信号肽位于成熟Amblin的N端，由15个氨基酸组成。由151个氨基酸组成的成熟Amblin含有14个半胱氨酸和两个类Kunitz结构域。它与来自肩突硬蜱的组织因子途径抑制剂（TFPI）Ixolaris具有高度的序列相似性，Ixolaris有10个半胱氨酸，还与来自微小牛蜱的凝血酶抑制剂Boophilin具有高度的序列相似性，Boophilin有12个半胱氨酸。重组Amblin对凝血酶的抑制作用与天然Amblin一样有效。这是关于蜱血淋巴中凝血酶抑制剂的首次报道。
