Kiris I G A, Eroldoğan O T, Kir M, Kumlu M
Faculty of Fisheries, Department of Aquaculture, Cukurova University, 01330, Balcali, Adana, Turkey.
Comp Biochem Physiol A Mol Integr Physiol. 2004 Oct;139(2):239-44. doi: 10.1016/j.cbpb.2004.09.008.
The effects of neuropeptide Y (NPY) administered intramuscularly or orally on postlarvae (PLs) of two penaeid species were investigated in this study. In experiment 1, food intake (FI) of Marsupenaeus japonicus PLs (0.96 g), injected with NPY at 0.6 microg per g BW, was investigated within 48 h posttreatment. In experiment 2, oral administration of NPY (at doses of 0, 0.125, 0.25, 0.5 microg g(-1) food) on feed intake and growth performance of Penaeus semisulcatus PLs (0.27 g) was examined for 6 weeks. In experiment 1, NPY injection significantly increased average daily FI of M. japonicus PLs within the first 24 h compared to the control (P<0.05), but its stimulatory effect decreased on the second day (P>0.05). The increase in FI was 33% during the first 24 h and 17% during the next 24 h. In experiment 2, significant (P<0.05) differences were found among the groups in terms of weight gain and food utilization (P<0.05). Mean FI significantly increased (as much as 1.3-fold over the control) when NPY was orally administered at doses from 0.125 to 0.5 microg g(-1) feed. There was a positive relationship between FI and final weight (y=-0.972+2.098x, R(2)=0.81) and between FCE and NPY doses in the diets (y=45.37+3.46x, R(2)=0.91). The present findings indicated for the first time that NPY is a potent stimulator of food intake when administered either intramuscularly or orally to penaeid shrimps.
本研究调查了肌肉注射或口服神经肽Y（NPY）对两种对虾后期幼体（PL）的影响。在实验1中，研究了以每克体重0.6微克的剂量给日本囊对虾PL（0.96克）注射NPY后48小时内的摄食量（FI）。在实验2中，研究了对凡纳滨对虾PL（0.27克）口服NPY（剂量为0、0.125、0.25、0.5微克/克饲料）对其摄食和生长性能的影响，为期6周。在实验1中，与对照组相比，注射NPY后日本囊对虾PL在最初24小时内的平均日摄食量显著增加（P<0.05），但第二天其刺激作用减弱（P>0.05）。最初24小时内摄食量增加了33%，接下来24小时内增加了17%。在实验2中，各实验组在体重增加和食物利用率方面存在显著差异（P<0.05）。当以0.125至0.5微克/克饲料的剂量口服NPY时，平均摄食量显著增加（比对照组高1.3倍）。摄食量与最终体重之间呈正相关（y=-0.972+2.098x，R²=0.81），饲料转化率与饲料中NPY剂量之间也呈正相关（y=45.37+3.46x，R²=0.91）。本研究结果首次表明，对虾肌肉注射或口服NPY时，它是一种有效的摄食刺激剂。
