Shun Cheng, Mei-Li Chi, Jian-Bo Zheng, Wen-Ping Jiang, Shi-Li Liu, Xiao-Ying Hang, Miao Peng, Fei Li, Dan-Li Wang
Zhejiang Institute of Freshwater Fisheries Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou, Zhejiang 313001, China.
School of Marine Sciences Ningbo University, Ningbo, Zhejiang 315211, China.
Aquac Nutr. 2024 Aug 24;2024:8347388. doi: 10.1155/2024/8347388. eCollection 2024.
To further explore the impact of nutrient fortification on the ovarian secondary development of redclaw crayfish, four groups were set up: group ①, no feeding; group ②, formulated feeding; groups ③ and ④, formulated feed + nutrient bait. Results showed (1) the proportions of egg-bearing shrimp in groups ③ and ④. 2) The weight growth rate of group ① was the lowest, the maturation coefficient of unripe shrimp in group ④ was the highest, and the egg-holding rates in groups ③ and ④ were significantly higher than those of group ①. The hatching and survival rates of yellow eggs in group ④ were significantly higher than those in group ②. The hatching and survival rates of red eggs in groups ③ and ④ were significantly higher than those in group ①. (3) The weight gain rate of juveniles in group ④ was significantly higher than those of group ①. (4) Genes and proteins related to ovarian development were screened. In summary, group ④ had a higher proportion of egg-holding shrimp, faster ovarian development, and a higher maturity coefficient. The hatching and survival rates of eggs, and the survival and weight gain rates of the offspring were also high.
为进一步探究营养强化对红螯螯虾卵巢二次发育的影响，设置了四组：①组，不投喂；②组，配合饲料投喂；③组和④组，配合饲料+营养饵料。结果显示：（1）③组和④组抱卵虾的比例。（2）①组的体重增长率最低，④组未成熟虾的成熟系数最高，③组和④组的抱卵率显著高于①组。④组黄卵的孵化率和存活率显著高于②组。③组和④组红卵的孵化率和存活率显著高于①组。（3）④组幼虾的增重率显著高于①组。（4）筛选出了与卵巢发育相关的基因和蛋白。综上所述，④组抱卵虾比例较高，卵巢发育较快，成熟系数较高。卵的孵化率和存活率以及幼体的存活率和增重率也较高。
