体外卵母细胞成熟用于治疗多囊卵巢综合征相关不孕症：法国的经验。

In vitro oocyte maturation for the treatment of infertility associated with polycystic ovarian syndrome: the French experience.

作者信息

Le Du A, Kadoch I J, Bourcigaux N, Doumerc S, Bourrier M-C, Chevalier N, Fanchin R, Chian R-C, Tachdjian G, Frydman R, Frydman N

机构信息

Service de Biologie et Génétique de la Reproduction, Obstétrique et Médecine de la Reproduction, Hôpital Antoine Béclère, Clamart, France.

出版信息

Hum Reprod. 2005 Feb;20(2):420-4. doi: 10.1093/humrep/deh603. Epub 2004 Nov 4.

DOI:10.1093/humrep/deh603

PMID:15528263

Abstract

BACKGROUND

In vitro oocyte maturation (IVM) permits the use of immature oocytes in IVF. IVM does not require ovarian stimulation and so can be offered to patients at risk of ovarian hyperstimulation syndrome.

METHODS

For this indication, we carried out 45 cycles of IVM in 33 women with polycystic ovarian syndrome (PCOS).

RESULTS

A total of 509 cumulus-oocyte complexes was obtained; 276 (54.2%) oocytes matured in 24 h and 45 (8.8%) in 48 h. The normal fertilization (2PN) rate of oocytes matured in 24 and 48 h was 69.5 and 73.3% respectively. Among the 214 embryos obtained, 103 were transferred and 30 were frozen. Forty transfers were performed (2.5 embryos/transfer). Eleven women had a positive beta-hCG test (26.2% of pregnancies/puncture, 27.5% of pregnancies/transfer) and nine women had a clinical pregnancy (20.0% of pregnancies/puncture, 22.5% of pregnancies/transfer). Five babies have been born and one pregnancy is ongoing. Results of the clinical examination carried out at birth were normal.

CONCLUSIONS

Our results show that IVM may be offered as an alternative to conventional IVF and to ovarian drilling in women with PCOS. The role of IVM in the therapeutic armamentarium for this condition should be further clarified.

摘要

背景

体外卵母细胞成熟（IVM）使得未成熟卵母细胞可用于体外受精（IVF）。IVM不需要卵巢刺激，因此可提供给有卵巢过度刺激综合征风险的患者。

方法

针对这一适应症，我们对33例多囊卵巢综合征（PCOS）女性进行了45个周期的IVM。

结果

共获得509个卵丘-卵母细胞复合体；276个（54.2%）卵母细胞在24小时内成熟，45个（8.8%）在48小时内成熟。在24小时和48小时成熟的卵母细胞正常受精（2PN）率分别为69.5%和73.3%。在获得的214个胚胎中，103个被移植，30个被冷冻。进行了40次移植（每次移植2.5个胚胎）。11名女性β-hCG检测呈阳性（占穿刺妊娠的26.2%，占移植妊娠的27.5%），9名女性临床妊娠（占穿刺妊娠的20.0%，占移植妊娠的22.5%）。已出生5名婴儿，1例妊娠仍在继续。出生时进行的临床检查结果正常。