潮间带微生物垫中细菌多样性和生理状态的联合磷脂生物标志物-16S rRNA基因变性梯度凝胶电泳分析
Combined phospholipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis of bacterial diversity and physiological status in an intertidal microbial mat.
作者信息
Villanueva Laura, Navarrete Antoni, Urmeneta Jordi, White David C, Guerrero Ricardo
机构信息
Department of Microbiology, University of Barcelona, Barcelona, Spain.
出版信息
Appl Environ Microbiol. 2004 Nov;70(11):6920-6. doi: 10.1128/AEM.70.11.6920-6926.2004.
Abstract
A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.
摘要
采用脂质生物标志物与16S rRNA基因变性梯度凝胶电泳相结合的分析方法，监测微生物席的生理状态、生物量和微生物组成的变化。在上午时段，观察到含有高密度光合生物的层生物量增加，而深层的生长速率下降。这种联合方法还揭示了主要微生物类群之间的差异，包括绿色非硫细菌、革兰氏阳性菌和异养细菌。
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