Department of Microbiology, University of Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria.
World J Microbiol Biotechnol. 2012 Feb;28(2):755-9. doi: 10.1007/s11274-011-0847-7. Epub 2011 Jul 17.
A considerable decline in viability of spray dried cells of Geotrichum klebahnii was observed and was attributed to an undefined alteration of the used strain. As common techniques were not able to distinguish the altered from the still viable strains, we used the fatty acid methyl ester (FAME) analysis. On the basis of FAME data we were able to discriminate the three strains under investigation. Especially the ratios of cis/trans fatty acid ratios and of saturated/unsaturated fatty acid were significantly reduced in the less viable strain, pointing to an increased stress level in this strain. These findings clearly show the applicability of the FAME analysis to detect strain alterations and that this method is therefore a suitable, fast and feasible tool for quality assurance.
我们观察到喷雾干燥的克利夫酵母细胞活力明显下降，并认为这是由于所用菌株的未知变化所致。由于常用技术无法区分已改变的和仍然具有活力的菌株，我们使用脂肪酸甲酯（FAME）分析。基于 FAME 数据，我们能够区分研究中的三种菌株。特别是在生存能力较低的菌株中，顺式/反式脂肪酸的比例和饱和/不饱和脂肪酸的比例明显降低，表明该菌株的应激水平增加。这些发现清楚地表明 FAME 分析可用于检测菌株变化，因此该方法是一种合适、快速且可行的质量保证工具。
