Ichimura Noriko, Shimojima Masayuki, Nishimura Yorihiro, Tohya Yukinobu, Akashi Hiroomi
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
J Vet Med Sci. 2004 Oct;66(10):1255-8. doi: 10.1292/jvms.66.1255.
Human CD7 is one of the earliest molecules to appear in T cell development. In this study, putative feline CD7 cDNA was identified based on its similarities with human and mouse CD7 genes. The feline CD7 cDNA contained an open reading frame consisting of 630 nucleotides. The amino acid sequence of feline CD7 had 47.7% identity with that of human CD7, and 52.9% with that of mouse CD7. In addition, the feline CD7 protein fused with histidine tag was expressed in 293T cells. The expression was confirmed by indirect immunofluorescence assay.
人类CD7是T细胞发育过程中最早出现的分子之一。在本研究中，基于与人类和小鼠CD7基因的相似性鉴定出了假定的猫CD7 cDNA。猫CD7 cDNA包含一个由630个核苷酸组成的开放阅读框。猫CD7的氨基酸序列与人类CD7的氨基酸序列有47.7%的同一性，与小鼠CD7的氨基酸序列有52.9%的同一性。此外，与组氨酸标签融合的猫CD7蛋白在293T细胞中表达。通过间接免疫荧光测定法证实了该表达。
