Du Jian, Zhang Sheng, Sun Rui, Zhang Li-Fang, Xiong Cheng-Dong, Peng Yu-Xing
Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, People's Republic of China.
J Biomed Mater Res B Appl Biomater. 2005 Feb 15;72(2):299-304. doi: 10.1002/jbm.b.30156.
Carboxymethyl konjac glucomannan-chitosan (CKGM-CS) nanoparticles were spontaneously prepared under very mild conditions via polyelectrolyte complexation. Bovine serum albumin (BSA), as a model protein drug, was incorporated into the CKGM-CS nanoparticles. The physicochemical properties of the BSA-loaded nanoparticles were identified by Zetasizer 3000 and FTIR spectrophotometry. Their sizes were from 330 nm to 900 nm; zeta potentials were positive according to varies CKGM/CS ratios. The encapsulation efficiency was up 20%. The release behavior in vitro of BSA from the nanoparticles was also investigated. We could find that the BSA release from the CKGM-CS nanoparticles is much more influenced by the CS coating layer than by the CKGM inner structure. And the CKGM-CS matrices not only exhibited pH-responsive properties, but ionic strength-sensitive properties. These systems may present a potential for pulsatile protein drug delivery.
羧甲基魔芋葡甘聚糖-壳聚糖（CKGM-CS）纳米颗粒通过聚电解质络合在非常温和的条件下自发制备。牛血清白蛋白（BSA）作为一种模型蛋白药物，被包封到CKGM-CS纳米颗粒中。通过Zetasizer 3000和傅里叶变换红外光谱法对负载BSA的纳米颗粒的物理化学性质进行了鉴定。它们的尺寸在330纳米至900纳米之间；根据不同的CKGM/CS比例，zeta电位为正值。包封效率高达20%。还研究了纳米颗粒中BSA的体外释放行为。我们发现，CKGM-CS纳米颗粒中BSA的释放受CS包衣层的影响远大于CKGM内部结构。并且CKGM-CS基质不仅表现出pH响应特性，还表现出离子强度敏感特性。这些体系可能具有脉动式蛋白药物递送的潜力。
