Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, China.
Biotechnol J. 2013 Jul;8(7):847-54. doi: 10.1002/biot.201300093. Epub 2013 Jun 7.
Polymeric nanoparticles have emerged as a promising approach for drug delivery systems. We prepared chitosan (CS)/sodium alginate (SAL) polyelectrolyte complex nanoparticles (CS/SAL NPs) via a simple and mild ionic gelation method by adding a CS solution to a SAL solution, and investigated the effects of molecular weight of the added CS, and the SAL:CS mass ratio on the formation of the polyelectrolyte complex nanoparticles. The well-defined CS/SAL NPs with near-monodisperse particle size of about 160 nm exhibited a pH stable structure, and pH responsive properties with a negatively or positively charged surface. The so-called "electrostatic sponge" structure of the polyelectrolyte complex nanoparticles enhanced their drug-loading capacity towards the differently charged model drug molecules, and favored controlled release. We also found that the drug-loading capacity was influenced by the nature of the drugs and the drug-loading media, while drug release was affected by the solubility of the drugs in the drug-releasing media. The biocompatibility and biodegradability of the polyelectrolytes in the polyelectrolyte complex nanoparticles were maintained by ionic interactions. These results indicate that CS/SAL NPs can represent a useful technique for pH-responsive drug delivery systems.
聚合物纳米粒子已成为药物传递系统的一种有前途的方法。我们通过向海藻酸钠（SAL）溶液中添加壳聚糖（CS）溶液，通过简单温和的离子凝胶化方法制备了壳聚糖（CS）/海藻酸钠（SAL）聚电解质复合纳米粒子（CS/SAL NPs），并研究了添加 CS 的分子量以及 SAL：CS 质量比对聚电解质复合纳米粒子形成的影响。具有约 160nm 近单分散粒径的明确定义的 CS/SAL NPs 具有 pH 稳定结构和 pH 响应特性，具有负电荷或正电荷表面。聚电解质复合纳米粒子的所谓“静电海绵”结构增强了它们对不同带电模型药物分子的药物负载能力，并有利于控制释放。我们还发现，药物负载能力受药物性质和药物负载介质的影响，而药物释放受药物在释放介质中的溶解度的影响。离子相互作用保持了聚电解质复合纳米粒子中聚电解质的生物相容性和可生物降解性。这些结果表明，CS/SAL NPs 可以代表 pH 响应药物传递系统的一种有用技术。
