Zuberovic Aida, Ullsten Sara, Hellman Ulf, Markides Karin E, Bergquist Jonas
Department of Analytical Chemistry, Biomedical Centre, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden.
Rapid Commun Mass Spectrom. 2004;18(23):2946-52. doi: 10.1002/rcm.1712.
Capillary electrophoresis (CE) was coupled off-line with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) for the analysis of proteins and peptides. CE fractions were collected directly on a matrix-coated MALDI target, using a sheath-flow interface. Protein adsorption during CE separations was prevented by coating the capillaries with the physically adsorbed, cationic polymer PolyE-323. The CE/MALDI-MS system was used for the analysis of model proteins and peptides at physiological pH as well as analysis of proteins in tear fluid. Moreover, tryptic on-target digestion of the collected protein fractions, with subsequent MALDI-MS and MS/MS peptide analysis, was demonstrated.
毛细管电泳（CE）与基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）离线联用，用于蛋白质和肽的分析。使用鞘流接口将CE馏分直接收集在涂有基质的MALDI靶上。通过用物理吸附的阳离子聚合物PolyE-323涂覆毛细管，可防止CE分离过程中的蛋白质吸附。CE/MALDI-MS系统用于在生理pH下分析模型蛋白质和肽，以及分析泪液中的蛋白质。此外，还展示了对收集的蛋白质馏分进行胰蛋白酶靶上消化，随后进行MALDI-MS和MS/MS肽分析。
