Lomada Dakshayani, Gambhira Ratish, Nehete Pramod N, Guhad Faisal A, Chopra Ashok K, Peterson Johnny W, Sastry K Jagannadha
Department of Veterinary Sciences, M.D. Anderson Cancer Center, The University of Texas, Bastrop, TX 78602, USA.
Vaccine. 2004 Dec 9;23(4):555-65. doi: 10.1016/j.vaccine.2004.05.039.
Vaccination with peptide antigens is an effective strategy against mucosal viral infections. We tested a two-codon mutant of cholera toxin (CT-2*) lacking ADP-ribosylating activity and toxicity as a mucosal adjuvant for T cell epitope peptides for intranasal immunization of mice. Efficient induction of helper and cytotoxic T lymphocyte responses associated with TH1 cytokine production were observed in the systemic and mucosal compartments including nasal, gut, and vaginal associated lymphoid tissues. Single or multiple dosing with the peptide antigen and CT-2* induced strong memory immunity without tolerance. These results demonstrate CT-2* as a suitable mucosal adjuvant for priming antigen-specific cellular immune responses.
用肽抗原进行疫苗接种是对抗粘膜病毒感染的有效策略。我们测试了一种缺乏ADP-核糖基化活性和毒性的霍乱毒素双密码子突变体（CT-2*），作为用于小鼠鼻内免疫的T细胞表位肽的粘膜佐剂。在包括鼻、肠道和阴道相关淋巴组织在内的全身和粘膜区室中，观察到与TH1细胞因子产生相关的辅助性和细胞毒性T淋巴细胞反应的有效诱导。用肽抗原和CT-2进行单次或多次给药可诱导强烈的记忆免疫而无耐受性。这些结果证明CT-2是引发抗原特异性细胞免疫反应的合适粘膜佐剂。
