Ishiguro Kazuhiro, Cao Zhifang, Ilasca Marco Lopez, Ando Takafumi, Xavier Ramnik
Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA.
Exp Cell Res. 2004 Dec 10;301(2):331-7. doi: 10.1016/j.yexcr.2004.09.003.
WAVE2 is a member of the WASP/WAVE family of protein effectors of actin reorganization and cell movement. In this report, we demonstrate that WAVE2 overexpression induces serum response element (SRE) activation through serum response factor. A WAVE2 mutant lacking the VCA region did not induce SRE activation and actin polymerization. WAVE2-induced SRE activation was blocked by exposure of cells to Latrunculin A, or overexpression of actin mutant R62D. The DeltaVCA mutant inhibited Rac V12-induced SRE activation, suggesting that WAVE2 lies downstream of Rac. Similar deletion of the VCA domain of WASP attenuated Cdc42 V12-mediated SRE activation, suggesting that WAVE2 acts in relation to Rac as WASP acts in relation to Cdc42. WAVE2 overexpression did not activate NF-kappaB.
WAVE2是肌动蛋白重组和细胞运动的WASP/WAVE家族蛋白质效应器的成员。在本报告中，我们证明WAVE2过表达通过血清反应因子诱导血清反应元件（SRE）激活。缺乏VCA区域的WAVE2突变体不会诱导SRE激活和肌动蛋白聚合。将细胞暴露于Latrunculin A或过表达肌动蛋白突变体R62D可阻断WAVE2诱导的SRE激活。DeltaVCA突变体抑制Rac V12诱导的SRE激活，表明WAVE2位于Rac的下游。WASP的VCA结构域的类似缺失减弱了Cdc42 V12介导的SRE激活，表明WAVE2与Rac的关系如同WASP与Cdc42的关系。WAVE2过表达不会激活NF-κB。
