Amézcua-Vega C, Ferrera-Cerrato R, Esparza-García F, Ríos-Leal E, Rodríguez-Vázquez R
Depto. de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, A.P., Col. San Pedro Zacatenco, C.P., México D.F.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2004;39(11-12):2983-91.
This work investigated biosurfactant production by Pseudomonas putida in combined C/P, C/Ninorganic, C/Fe, C/Mg nutrient ratios and peptone concentration. Analysis of the 2(5-1) fractional factorial experimental design showed that only the C/Fe ratio had a significant (p<0.02) effect on biosurfactant production. The highest amount of biosurfactant was obtained at low C/Fe ratios, but net surface tension did not show significant differences. In addition, low amounts of peptone and the C/P-C/Mg nutrient ratios interaction significantly (p < 0.05) enhanced the biomass produced by P. putida. Analysis of biosurfactant by gas chromatography (GC) showed that the hydrophilic fraction was composed by rhamnose and the hydrophobic fraction, mainly by palmitic (C16), stearic (C18:0), oleic (C18:1) and linoleic (C18:2) fatty acids.
本研究调查了恶臭假单胞菌在不同碳/磷、碳/无机氮、碳/铁、碳/镁营养比以及蛋白胨浓度条件下生物表面活性剂的产生情况。对2(5 - 1)部分因子实验设计的分析表明，只有碳/铁比（p<0.02）对生物表面活性剂的产生有显著影响。在低碳/铁比条件下获得了最高量的生物表面活性剂，但净表面张力没有显著差异。此外，低量的蛋白胨以及碳/磷 - 碳/镁营养比之间的相互作用显著（p < 0.05）提高了恶臭假单胞菌产生的生物量。通过气相色谱（GC）对生物表面活性剂的分析表明，亲水部分由鼠李糖组成，疏水部分主要由棕榈酸（C16）、硬脂酸（C18:0）、油酸（C18:1）和亚油酸（C18:2）脂肪酸组成。
