Ivanov Andrej I, Rovescalli Alessandra C, Pozzi Paola, Yoo Siuk, Mozer Brian, Li Hsi-Ping, Yu Shu-Hua, Higashida Haruhiro, Guo Vicky, Spencer Michael, Nirenberg Marshall
Laboratory of Biochemical Genetics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16216-21. doi: 10.1073/pnas.0407188101. Epub 2004 Nov 8.
RNA interference was used to screen 3,314 Drosophila double-stranded RNAs, corresponding to approximately 25% of Drosophila genes, for genes that affect the development of the embryonic nervous system. RNA-interference-mediated gene silencing in Drosophila embryos resulted in loss-of-function mutant phenotypes for 43 genes, which is 1.3% of the genes that were screened. We found 18 genes that were not known previously to affect the development of the nervous system. The functions of some of the genes are unknown. Other genes encode protein kinases, transcription factors, and signaling proteins, as well as proteins with other functions.
利用RNA干扰技术对3314条果蝇双链RNA（对应约25%的果蝇基因）进行筛选，以寻找影响胚胎神经系统发育的基因。果蝇胚胎中RNA干扰介导的基因沉默导致43个基因出现功能丧失突变表型，占所筛选基因的1.3%。我们发现了18个以前未知会影响神经系统发育的基因。其中一些基因的功能尚不清楚。其他基因编码蛋白激酶、转录因子、信号蛋白以及具有其他功能的蛋白。
