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PTEN inhibits cell proliferation and induces apoptosis by downregulating cell surface IGF-IR expression in prostate cancer cells.PTEN通过下调前列腺癌细胞表面IGF-IR的表达来抑制细胞增殖并诱导细胞凋亡。
Oncogene. 2004 Jan 22;23(3):786-94. doi: 10.1038/sj.onc.1207162.
2
PTEN mutations are common in sporadic microsatellite stable colorectal cancer.PTEN突变在散发性微卫星稳定型结直肠癌中很常见。
Oncogene. 2004 Jan 15;23(2):617-28. doi: 10.1038/sj.onc.1207059.
3
[Detection of mutation and protein expression of PTEN gene in endometrial carcinoma].子宫内膜癌中PTEN基因的突变及蛋白表达检测
Ai Zheng. 2004 Jan;23(1):69-73.
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PTEN gene mutation and high MIB-1 labeling index may contribute to dissemination in patients with glioblastoma.PTEN基因突变和高MIB-1标记指数可能促使胶质母细胞瘤患者发生播散。
J Clin Neurosci. 2004 Jan;11(1):37-41. doi: 10.1016/j.jocn.2002.09.001.
5
Genetic interactions between Pten and p53 in radiation-induced lymphoma development.Pten与p53在辐射诱导的淋巴瘤发生中的遗传相互作用。
Oncogene. 2003 Nov 20;22(52):8379-85. doi: 10.1038/sj.onc.1207083.
6
Adenovirus-mediated PTEN treatment combined with caffeine produces a synergistic therapeutic effect in colorectal cancer cells.腺病毒介导的PTEN治疗联合咖啡因对结肠癌细胞产生协同治疗效果。
Cancer Gene Ther. 2003 Nov;10(11):803-13. doi: 10.1038/sj.cgt.7700644.
7
PTEN expression in PTEN-null leukaemic T cell lines leads to reduced proliferation via slowed cell cycle progression.在PTEN基因缺失的白血病T细胞系中，PTEN表达通过减缓细胞周期进程导致增殖减少。
Oncogene. 2003 Nov 6;22(50):8195-204. doi: 10.1038/sj.onc.1206872.
8
Expression of PTEN in renal cell carcinoma and its relation to tumor behavior and growth.PTEN在肾细胞癌中的表达及其与肿瘤行为和生长的关系。
J Surg Oncol. 2003 Nov;84(3):166-72. doi: 10.1002/jso.10302.
9
Adenovirus-mediated transfer of the PTEN gene inhibits human colorectal cancer growth in vitro and in vivo.腺病毒介导的PTEN基因转移在体外和体内均可抑制人结直肠癌的生长。
Gene Ther. 2003 Nov;10(23):1961-9. doi: 10.1038/sj.gt.3302100.
10
Suppression of cell migration in ovarian cancer cells mediated by PTEN overexpression.PTEN过表达介导的卵巢癌细胞迁移抑制
Int J Oncol. 2003 Oct;23(4):1109-13.

转染的PTEN对人结肠癌的抑制作用。

Inhibition of transfected PTEN on human colon cancer.

作者信息

Xu Shou-Shui, Shen Wen-Lu, Ouyang Song-Ying

机构信息

Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, Guangdong Province, China.

出版信息

World J Gastroenterol. 2004 Dec 15;10(24):3670-3. doi: 10.3748/wjg.v10.i24.3670.

DOI:10.3748/wjg.v10.i24.3670
PMID:15534929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4612015/
Abstract

AIM

To study the inhibitory effect of transfected PTEN on LoVo cells.

METHODS

Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by flow cytometry, DNA bands and MTT.

RESULTS

PTEN transfection significantly up-regulated PTEN expression in LoVo cells. 5-Fu inhibited cell proliferation and induced apoptosis in transfected LoVo cells.

CONCLUSION

Transfected PTEN can remarkably up-regulate PTEN expression in LoVo cells and promote the apoptosis. PTEN transfection is associated with 5-Fu treatment effect and has a cooperatively cytotoxic effect.

摘要

目的

研究转染PTEN对LoVo细胞的抑制作用。

方法

通过脂质体将人PTEN cDNA导入LoVo细胞，采用蛋白质免疫印迹法和流式细胞术分析PTEN mRNA水平及其表达。转染前后，采用流式细胞术、DNA条带分析和MTT法检测5-氟尿嘧啶对细胞增殖的抑制作用和诱导凋亡的作用。

结果

PTEN转染显著上调了LoVo细胞中PTEN的表达。5-氟尿嘧啶抑制转染的LoVo细胞的增殖并诱导其凋亡。

结论

转染的PTEN可显著上调LoVo细胞中PTEN的表达并促进凋亡。PTEN转染与5-氟尿嘧啶的治疗效果相关，具有协同细胞毒性作用。