Boss P, Monckton R P, Nicolet J, Burnens A P
Institut für Veterinärbakteriologie, Universität Bern.
Schweiz Arch Tierheilkd. 1992;134(1):31-7.
We tested hemolytic E. coli from 86 pigs with edema disease or colidiarrhea. They were tested serologically and with nonradioactive digoxigenin-dUTP labelled probes for the presence of enterotoxin or Shiga-like-toxin genes. By slide-agglutination we detected 38 cases with E. coli O149:K88, 28 with E. coli O139:82B and 20 with E. coli O141. E. coli of serogroup O149:K88 isolated from diarrheic pigs, reacted with the probes for LT and STb genes. Edema disease E. coli O139:82B reacted with the SLTII probe. E. coli O141, isolated from colidiarrhea or edema disease showed a diversity of toxin gene patterns. All the E. coli O141 from diarrheic pigs reacted with the probes for LT and STap in addition to SLTII. No strains isolated from pigs with edema disease possessed any of these enterotoxin genes. Gene probe technique confirmed the serological method as useful tool for diagnosing E. coli O149:K88 and O139:82B as ETEC or VTEC, respectively. On the other hand only the demonstration of toxin genes with probes could explain the pathological findings in the pigs shedding E. coli of serogroup O141.
我们检测了86头患有水肿病或结肠腹泻的猪身上的溶血性大肠杆菌。对它们进行了血清学检测，并使用非放射性地高辛配基-dUTP标记探针检测是否存在肠毒素或志贺样毒素基因。通过玻片凝集试验，我们检测到38例大肠杆菌O149:K88、28例大肠杆菌O139:82B和20例大肠杆菌O141。从腹泻猪中分离出的血清群O149:K88大肠杆菌与LT和STb基因探针发生反应。水肿病大肠杆菌O139:82B与SLTII探针发生反应。从结肠腹泻或水肿病中分离出的大肠杆菌O141表现出多种毒素基因模式。所有从腹泻猪中分离出的大肠杆菌O141除了与SLTII反应外，还与LT和STap探针发生反应。从患有水肿病的猪中分离出的菌株均不具有任何这些肠毒素基因。基因探针技术证实血清学方法是分别诊断大肠杆菌O149:K88和O139:82B为产肠毒素大肠杆菌（ETEC）或产志贺毒素大肠杆菌（VTEC）的有用工具。另一方面，只有用探针证明毒素基因才能解释排出血清群O141大肠杆菌的猪的病理发现。
