Reynolds B A, Weiss S
Department of Pathology, University of Calgary Faculty of Medicine, Alberta, Canada.
Science. 1992 Mar 27;255(5052):1707-10. doi: 10.1126/science.1553558.
Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth; in the mouse striatum no new neurons are produced after the first few days after birth. In this study, cells isolated from the striatum of the adult mouse brain were induced to proliferate in vitro by epidermal growth factor. The proliferating cells initially expressed nestin, an intermediate filament found in neuroepithelial stem cells, and subsequently developed the morphology and antigenic properties of neurons and astrocytes. Newly generated cells with neuronal morphology were immunoreactive for gamma-aminobutyric acid and substance P, two neurotransmitters of the adult striatum in vivo. Thus, cells of the adult mouse striatum have the capacity to divide and differentiate into neurons and astrocytes.
哺乳动物中枢神经系统中的神经发生被认为在出生后不久就结束了；在小鼠纹状体中，出生后头几天之后就不再产生新的神经元。在本研究中，从成年小鼠脑纹状体分离出的细胞在体外被表皮生长因子诱导增殖。增殖细胞最初表达巢蛋白，这是一种在神经上皮干细胞中发现的中间丝，随后发展出神经元和星形胶质细胞的形态及抗原特性。具有神经元形态的新生成细胞对γ-氨基丁酸和P物质呈免疫反应，这是成年体内纹状体的两种神经递质。因此，成年小鼠纹状体的细胞具有分裂并分化为神经元和星形胶质细胞的能力。
