Kristiansson Stefan, Juzeniene Asta, Juzenas Petras, Iani Vladimir, Löfgren Lennart, Moan Johan
Department of Otolaryngology, Orebro University Hospital, Orebro, Sweden.
Photochem Photobiol. 2005 Mar-Apr;81(2):394-7. doi: 10.1562/2004-04-02-RA-132.
The kinetics of accumulation of protoporphyrin IX (PpIX) after topical application of 5-aminolevulinic acid (ALA) and its methylester (5-aminolevulinic acid methylester [ALA-Me]) was studied on rat oral mucosa. The accumulation of PpIX in mucosa and skin after intravenous injection of ALA and ALA-Me was also studied. The elimination rate of PpIX was dependent on drug and dose as well as on administration route. Application of ALA on rat oral mucosa and skin caused a systemic effect with PpIX building up in remote skin sites not exposed to the drugs. No such systemic effect was seen after application of ALA-Me either in mucosa or on skin. Intravenous injection of the drugs (0.2 g/kg) leads to more fluorescence in the skin than topical application of the drug (20%). For mucosa, the opposite is true. Maximal PpIX fluorescence appeared later after application of high concentrations of the drugs (around 8 h for 5% and 20% wt/wt) than after application of low concentrations (around 3-5 h for 1% and 2% wt/wt).
在大鼠口腔黏膜上研究了局部应用5-氨基乙酰丙酸（ALA）及其甲酯（5-氨基乙酰丙酸甲酯[ALA-Me]）后原卟啉IX（PpIX）的蓄积动力学。还研究了静脉注射ALA和ALA-Me后PpIX在黏膜和皮肤中的蓄积情况。PpIX的消除率取决于药物、剂量以及给药途径。在大鼠口腔黏膜和皮肤上应用ALA会产生全身效应，PpIX在未接触药物的远端皮肤部位蓄积。在黏膜或皮肤上应用ALA-Me后未观察到这种全身效应。静脉注射药物（0.2 g/kg）比局部应用药物（20%）在皮肤中产生更多荧光。对于黏膜，情况则相反。应用高浓度药物（5%和2０%重量/重量，约8小时）后比应用低浓度药物（1%和2%重量/重量，约3-5小时）后出现最大PpIX荧光的时间更晚。
