Campos Emilia, Revilla Concepción, Chamorro Sonia, Alvarez Belén, Ezquerra Angel, Domínguez Javier, Alonso Fernando
CISA-INIA, Valdeolmos, 28130 Madrid, Spain.
Vet Res. 2004 Nov-Dec;35(6):625-33. doi: 10.1051/vetres:2004041.
The effect of classical swine fever (CSF) virus on some phenotypic and functional features of an established porcine aortic endothelial cell (AOC) line was investigated. AOC cells show most of the characteristics of primary endothelial cells, avoiding the alterations and senescence that these cells undergo after a few passages in culture. AOC cells were susceptible to CSF virus infection to a high degree, reaching 90% of CSF virus positive cells after 24 h of infection; however as with other porcine susceptible cells, no cytopathic effect could be observed. In these conditions none of the surface molecules studied, including SLA-II MHC antigens, adhesion or co-stimulatory molecules, were altered by virus infection after 24 or 48 h. Functionally CSF virus infection induced a decrease in the pro-coagulant activity of the AOC cells, determined by the increase in the clot formation time shown by the lysates of these cells. This contrasts with the increase observed in the expression of mRNA corresponding to IL-1 alpha and IL-6, two proinflammatory and pro-coagulant cytokines, in CSF virus-infected AOC cells.
研究了经典猪瘟（CSF）病毒对已建立的猪主动脉内皮细胞（AOC）系某些表型和功能特征的影响。AOC细胞表现出原代内皮细胞的大多数特征，避免了这些细胞在培养传代几次后所经历的改变和衰老。AOC细胞对CSF病毒高度敏感，感染24小时后CSF病毒阳性细胞达到90%；然而，与其他猪易感细胞一样，未观察到细胞病变效应。在这些条件下，所研究的表面分子，包括SLA-II MHC抗原、黏附分子或共刺激分子，在24或48小时后均未因病毒感染而改变。在功能上，CSF病毒感染导致AOC细胞促凝血活性降低，这由这些细胞裂解物显示的凝血形成时间增加所确定。这与CSF病毒感染的AOC细胞中对应于IL-1α和IL-6（两种促炎和促凝血细胞因子）的mRNA表达增加形成对比。
