Tang Qing-Hai, Zhang Yan-Ming, Xu Yan-Zhao, He Lei, Dai Chen, Sun Pei
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi 712100, China.
Vet Immunol Immunopathol. 2010 Feb 15;133(2-4):237-42. doi: 10.1016/j.vetimm.2009.07.005. Epub 2009 Jul 28.
Classical swine fever (CSF) caused by virulent strains of classical swine fever virus (CSFV) is a haemorrhagic disease of pigs, characterized by disseminated intravascular coagulation, thrombocytopenia and immunosuppression. The cell adhesion molecule, integrin beta3, plays a central role in maintaining and regulating vascular permeability. In view of the haemorrhagic pathology of the disease, the effect of CSFV infection on integrin beta3 expression was investigated using the swine umbilical vein endothelial cell (SUVEC) line, in conjunction with quantitative PCR and Western blotting techniques. Following infection, the expression levels of integrin beta3 were significantly up-regulated along with corresponding transcription levels. The infected endothelial cells adhered onto immobilized extracellular matrix (ECM) with more extensive spreading than that of the control, and such interaction was strongly inhibited by an anti-integrin beta3 monoclonal antibody (mAb). This study revealed the up-regulation of integrin beta3 in vascular endothelial cells by CSFV infection, and cell adhesion molecules of this kind possibly play an important role in the changes of haemostatic balance in haemorrhagic pathology of CSF.
由强毒力的经典猪瘟病毒（CSFV）引起的经典猪瘟（CSF）是猪的一种出血性疾病，其特征为弥散性血管内凝血、血小板减少和免疫抑制。细胞黏附分子整合素β3在维持和调节血管通透性方面发挥着核心作用。鉴于该疾病的出血性病理特征，利用猪脐静脉内皮细胞（SUVEC）系，并结合定量PCR和蛋白质印迹技术，研究了CSFV感染对整合素β3表达的影响。感染后，整合素β3的表达水平随相应转录水平显著上调。与对照组相比，被感染的内皮细胞以更广泛的铺展方式黏附于固定化的细胞外基质（ECM）上，并且这种相互作用被抗整合素β3单克隆抗体（mAb）强烈抑制。本研究揭示了CSFV感染可使血管内皮细胞中的整合素β3上调，并且这类细胞黏附分子可能在CSF出血性病理中止血平衡的变化中发挥重要作用。
