Le Grand Dominique, Saras Estelle, Blond Denis, Solsona Michel, Poumarat François
UMR Mycoplasmoses des Ruminants, Pathologie du Bétail, Ecole Nationale Vétérinaire de Lyon, 1 avenue Bourgelat, 69280 Marcy-l'Etoile, France.
Vet Res. 2004 Nov-Dec;35(6):635-49. doi: 10.1051/vetres:2004037.
DNA amplification techniques offer considerable promise for the identification of Mycoplasma mycoides cluster members. They avoid antigenic cross-reactivity and variability that hamper serological methods. Many sets of primers, specific of these different members and of Mycoplasma putrefaciens, have been proposed. To assess the reliability of some of these PCR tests in routine laboratory diagnostic use, 230 field strains supposed to belong to this group were simultaneously identified by PCR and an antigenic method. The results were well correlated to antigenic identification for M. putrefaciens, but PCR failed to identify respectively 74% and 52% of M. mycoides subsp. mycoides Large Colony type and M. capricolum subsp. capricolum strains. Any identification of M. mycoides subsp. mycoides Small Colony type must be confirmed by two different tests. Difficulties in defining the M. species bovine serogroup 7 were also encountered with both the PCR and immunological methods. The occurrence of putative variable antigen(s) on the mycoplasma surface may explain part of the identification difficulties encountered with the immunological methods.
DNA扩增技术为鉴定丝状支原体簇成员提供了可观的前景。它们避免了妨碍血清学方法的抗原交叉反应和变异性。已经提出了许多针对这些不同成员以及腐败支原体的引物组。为了评估其中一些PCR检测方法在常规实验室诊断中的可靠性，通过PCR和一种抗原方法同时鉴定了230株假定属于该组的田间菌株。对于腐败支原体，结果与抗原鉴定高度相关，但PCR分别未能鉴定出74%的丝状支原体亚种丝状支原体大菌落型菌株和52%的山羊支原体亚种山羊支原体菌株。丝状支原体亚种丝状支原体小菌落型的任何鉴定都必须通过两种不同的检测方法来确认。在使用PCR和免疫方法时，在界定牛血清群7支原体物种方面也遇到了困难。支原体表面假定可变抗原的出现可能解释了免疫方法中遇到的部分鉴定困难。
