巴基斯坦南部地区山羊中[亚种名称未给出]的临床病理及血清分子检测
Clinicopathological and Sero-Molecular Detection of subsp. in Goats in Southern Areas of Pakistan.
作者信息
Rehman Faiz Ur, Khan Farhan Anwar, Saeed Muhammad, Ali Mehboob, Khan Hayatullah, Ahmad Faisal, Ullah Qudrat
机构信息
College of Veterinary Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan.
Livestock and Dairy Development (Research), Peshawar, Khyber Pakhtunkhwa, Pakistan.
出版信息
Vet Med Int. 2022 Oct 3;2022:9508810. doi: 10.1155/2022/9508810. eCollection 2022.
Contagious caprine pleuropneumonia (CCPP) is a highly fatal infectious disease of goats, caused by subsp. (Mccp). This disease is causing huge economic losses to the goat industry in Pakistan. However, little is known about the epidemiology of CCPP, especially in the hard areas of Khyber Pakhtunkhwa (KP), Pakistan, despite having a huge population of goats. Therefore, this study aimed to elucidate sero-molecular epidemiology and pathology associated with Mccp infection in goats in southern areas of KP including Dera Ismail Khan (DI Khan), Bannu, Karak, and Kohat. A total of 200 (50 from each area) serum samples were collected from clinically infected goats, whereas 600 various samples (nasal swab = 50, pleural fluid = 50, lungs = 50 at each selected area of study) were collected from live goats showing respiratory clinical signs and dead/slaughter goats having lesions in the lungs/pleura. A commercial competitive ELISA kit confirmed anti-Mccp antibodies in altogether 17% of serum samples, while area-wise seroprevalence was recorded as follows: Kohat, 28%, Bannu, 18%, DI Khan, 14%, and Karak, 8%. Moreover, a total of 5.5% of samples collected from clinically positive live and dead goats for Mccp were found by species-specific PCR, whereas area-wise molecular prevalence of Mccp was found in 3% samples from Kohat, 7.33%, Bannu, 6%, Khan, 5.33%, and Karak, 3.33%. Of 400 clinically examined goats, 242 (60%) had nasal discharge, 207 (51%) had pyrexia, 50.75% (203) had coughing, 48.25% (193) had pneumonia, 23% (92) had lacrimation, 7.75% (31) had pneumonia with lacrimation, and 10 (2.5%) showed all signs. Of the total 200 dead/slaughtered goats, pleural fluid was found in 36 goats and consolidation and red hepatization were observed in 40 and 42 goats, respectively. The present study found the presence of prevailing Mccp strain in the goat population of the study area. The highest prevalence of Mccp was found in collected samples from Kohat by PCR. The highest seroprevalence of Mccp was found in serum samples collected from Kohat by ELISA.
山羊传染性胸膜肺炎（CCPP）是由丝状支原体山羊亚种（Mccp）引起的一种山羊高致死性传染病。该病给巴基斯坦的山羊产业造成了巨大经济损失。然而，尽管巴基斯坦开伯尔-普赫图赫瓦省（KP）山羊数量众多，但对CCPP的流行病学了解甚少，尤其是在该省的偏远地区。因此，本研究旨在阐明KP省南部地区（包括德拉伊斯梅尔汗（DI Khan）、班努、卡拉奇和科哈特）山羊中与Mccp感染相关的血清分子流行病学及病理学情况。从临床感染的山羊中总共采集了200份血清样本（每个地区50份），同时从表现出呼吸道临床症状的活羊以及肺部/胸膜有病变的死羊/屠宰羊中，在每个选定的研究区域采集了600份不同样本（鼻拭子=50份、胸水=50份、肺组织=50份）。一种商业竞争性ELISA试剂盒在总共17%的血清样本中检测到抗Mccp抗体，各地区的血清阳性率记录如下：科哈特，28%；班努，18%；DI Khan，14%；卡拉奇，8%。此外，通过种特异性PCR检测发现，从临床确诊感染Mccp的活羊和死羊中采集的样本中，Mccp的总体阳性率为5.5%，各地区Mccp的分子阳性率分别为：科哈特3%、班努7.33%、汗5.33%、卡拉奇3.33%。在400只接受临床检查的山羊中，242只（60%）有鼻液，207只（51%）发热，203只（50.75%）咳嗽，193只（48.25%）有肺炎，92只（23%）流泪，31只（7.75%）有肺炎且流泪，10只（2.5%）出现所有症状。在总共200只死羊/屠宰羊中，36只羊有胸水，40只羊观察到肺实变，42只羊观察到红色肝变。本研究发现研究区域的山羊群体中存在流行的Mccp菌株。通过PCR检测发现，科哈特采集的样本中Mccp阳性率最高。通过ELISA检测发现，科哈特采集的血清样本中Mccp血清阳性率最高。
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