Dong Hongwei, Wang Quanxin, Valkova Katia, Gonchar Yuri, Burkhalter Andreas
Department of Anatomy and Neurobiology, Washington University School of Medicine, 8108 660 South Euclid Avenue, St. Louis, MO 63110, USA.
Vision Res. 2004 Dec;44(28):3389-400. doi: 10.1016/j.visres.2004.09.007.
Using whole cell recordings, we studied excitatory and inhibitory postsynaptic currents (EPSCs, IPSCs) in feedforward (FF) and feedback (FB) circuits between areas V1 and LM (lateromedial) in developing mouse visual cortex. We found that in mice reared with normal visual experience, FF and FB synapses onto layer 2/3 pyramidal neurons develop equal but submaximal strengths whose EPSCs are increased by monocular lid suture. In contrast, the development and experience-dependence of FF- and FB-IPSCs is pathway-specific. The difference develops during the critical period by strengthening FF-IPSCs, while keeping FB-IPSC amplitudes constant. Monocular lid suture increases FB-IPSCs but does not affect FF-IPSCs.
我们利用全细胞记录技术，研究了发育中小鼠视觉皮层V1区和LM（外侧内侧）区之间前馈（FF）和反馈（FB）回路中的兴奋性和抑制性突触后电流（EPSC、IPSC）。我们发现，在具有正常视觉经验的饲养小鼠中，投射到第2/3层锥体神经元上的FF和FB突触形成了相等但未达到最大值的强度，其EPSC通过单眼眼睑缝合而增加。相比之下，FF-IPSC和FB-IPSC的发育及经验依赖性具有通路特异性。这种差异在关键期通过增强FF-IPSC而形成，同时保持FB-IPSC的幅度不变。单眼眼睑缝合增加了FB-IPSC，但不影响FF-IPSC。
