Pan Chin-Chen, Chen Paul Chih-Hsueh, Chiang Hung
Department of Pathology, National Yang-Ming University and Veterans General Hospital, Taipei, Taiwan.
Appl Immunohistochem Mol Morphol. 2004 Dec;12(4):370-2. doi: 10.1097/00129039-200412000-00015.
Tissue array technique is a powerful tool for high-throughput in situ analysis on a large cohort of cases. This report describes an easy and effective method for manual construction of high-density tissue array containing 88 (11 x 8) samples, each of which measures 2 mm in diameter. No extraneous device is needed except a conventional 16-gauge bone marrow biopsy trephine apparatus to puncture the paraffin blocks. The authors constructed 563 cases of epithelial neoplasm into 7 blocks. The sectioning is smooth and does not require adhesive tape. They performed immunohistochemical staining for cytokeratins 7 and 20 on these tissue array slides. The samples rarely fell off during the antigen retrieval and staining procedure. The results were generally in agreement with those in previous reports. The authors offer a satisfactory alternative method for building custom arrays for any laboratory that is unable to afford a tissue array apparatus.
组织芯片技术是对大量病例进行高通量原位分析的有力工具。本报告描述了一种简便有效的手工构建高密度组织芯片的方法，该芯片包含88个（11×8）样本，每个样本直径为2毫米。除了用传统的16号骨髓活检环钻装置穿刺石蜡块外，无需其他额外设备。作者将563例上皮性肿瘤病例构建成7个组织块。切片平滑，无需胶带。他们对这些组织芯片载玻片进行了细胞角蛋白7和20的免疫组织化学染色。在抗原修复和染色过程中，样本很少脱落。结果与先前报道基本一致。作者为任何无力购买组织芯片设备的实验室提供了一种令人满意的定制芯片构建替代方法。
