使用简单设备构建和修复组织微阵列的简单方法。
Simple method for constructing and repairing tissue microarrays using simple equipment.
作者信息
Zhang Wei, Qiu Fuman, Jiang Qingping, Liu Shaoyan, Xiong Zhongtang
机构信息
Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.
State Key Lab of Respiratory Disease, Institute for Chemical Carcinogenesis, Collaborative Innovation Center for Environmental Toxicity, Guangzhou Medical University, Guangzhou, China.
出版信息
J Int Med Res. 2021 Mar;49(3):3000605211000156. doi: 10.1177/03000605211000156.
OBJECTIVE
Many methods for tissue microarray (TMA) construction were described in previous reports. Because TMA-based methods are expensive and complicated, their widespread application may be restricted. This study aimed to develop a simple method for TMA construction.
METHODS
High-density TMAs were constructed using simple equipment, and hematoxylin and eosin and immunohistochemical staining were performed to analyze the effect on the TMA block.
RESULTS
A recipient block with 162 holes of 0.9 mm in diameter was prepared using a mini-drill and plastic mold. Tissue cores of 1.0 mm in diameter were obtained from multiple donor blocks with stainless-steel capillary tubes driven by the mini-drill. Under the fixation and guidance of the plastic mold, tissue cores could be easily injected into the holes in the recipient block by inserting a stainless-steel wire into the stainless-steel tube with the tissue core and then pressing using the stainless-steel wire.
CONCLUSION
A high-density TMA block with 162 1.0-mm cores was created. This new modified technique could be a good alternative in many laboratories.
目的
先前的报告中描述了许多构建组织微阵列（TMA）的方法。由于基于TMA的方法昂贵且复杂，其广泛应用可能受到限制。本研究旨在开发一种构建TMA的简单方法。
方法
使用简单设备构建高密度TMA，并进行苏木精-伊红染色和免疫组织化学染色以分析对TMA块的影响。
结果
使用微型钻头和塑料模具制备了一个具有162个直径为0.9毫米孔的受体块。通过微型钻头驱动不锈钢毛细管从多个供体块中获取直径为1.0毫米的组织芯。在塑料模具的固定和引导下，通过将带有组织芯的不锈钢丝插入不锈钢管，然后用不锈钢丝按压，可轻松将组织芯注入受体块的孔中。
结论
创建了一个具有162个1.0毫米芯的高密度TMA块。这种新的改良技术在许多实验室中可能是一个很好的替代方法。
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