Moriwaki Hiroshi, Harino Hiroya, Yoshikura Taro, Ohe Takeshi, Nukaya Haruo, Terao Yoshiyasu, Sawanishi Hiroyuki, Wakabayashi Keiji, Miyakoda Hidekazu, Alary Jean-François
Osaka City Institute of Public Health & Environmental Sciences, 8-34, Tojo-cho, Tennoji-ku, Osaka 543-0026, Japan.
J Environ Monit. 2004 Nov;6(11):897-902. doi: 10.1039/b403965n. Epub 2004 Oct 14.
We describe a method for the simultaneous determination of eight kinds of phenylbenzotriazole-type mutagens (PBTA-1, -2, -3, -4, -5, -6, -7 and -8) in river water based on liquid chromatography-tandem mass spectrometry (LC/MS/MS). The application of dopant-assisted atmospheric pressure photoionization (APPI) for the detection of the PBTAs was studied. The APPI technique provided higher PBTA signal intensities than those obtained with an electrospray ionization (ESI) source, and the APPI method was used for the determination of the PBTAs. A solid-phase extraction procedure was used for the extractions of PBTA-1 through -8 from river water. The procedure was rapid and the relative standard deviations were below 15%. The detection limits of PBTA-1 through -8 in river water using the proposed method were found to range from 0.04 to 0.5 ng L(-1) and PBTAs were successfully detected in river water at sub-ng L(-1) levels.
我们描述了一种基于液相色谱 - 串联质谱法（LC/MS/MS）同时测定河水中八种苯基苯并三唑类诱变剂（PBTA - 1、 - 2、 - 3、 - 4、 - 5、 - 6、 - 7和 - 8）的方法。研究了掺杂剂辅助大气压光电离（APPI）在检测PBTA中的应用。与电喷雾电离（ESI）源相比，APPI技术提供了更高的PBTA信号强度，并将APPI方法用于PBTA的测定。采用固相萃取程序从河水中萃取PBTA - 1至 - 8。该程序快速，相对标准偏差低于15%。使用所提出的方法，河水中PBTA - 1至 - 8的检测限为0.04至0.5 ng L⁻¹，并且在河水中成功检测到了亚ng L⁻¹水平的PBTA。
