Kim J, Jung K, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-dong, Kwanak-Gu 151-742, Seoul, Republic of Korea.
Vet Rec. 2004 Oct 16;155(16):489-92. doi: 10.1136/vr.155.16.489.
A retrospective study of natural cases of abortion, recorded between October 2000 and September 2002, was made to determine the prevalence of abortions associated with porcine circovirus type 2 (PCV-2). The virus was detected by PCR, immunohistochemistry and in situ hybridisation. A total of 46 (13.1 per cent) of 350 aborted fetuses and stillborn piglets were positive for PCV-2 by PCR, and the virus was detected in fetuses at all stages of gestation. Viral antigen was detected in macrophages from the aborted fetuses and stillborn piglets by immunohistochemistry, and viral DNA was detected by in situ hybridisation.
为确定与2型猪圆环病毒(PCV - 2)相关的流产发生率,对2000年10月至2002年9月间记录的自然流产病例进行了回顾性研究。通过聚合酶链反应(PCR)、免疫组织化学和原位杂交检测病毒。在350例流产胎儿和死产仔猪中,共有46例(13.1%)经PCR检测PCV - 2呈阳性,且在妊娠各阶段的胎儿中均检测到该病毒。通过免疫组织化学在流产胎儿和死产仔猪的巨噬细胞中检测到病毒抗原,通过原位杂交检测到病毒DNA。