Park J-S, Kim J, Ha Y, Jung K, Choi C, Lim J-K, Kim S-H, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Kwanak-Gu 151-742 Seoul, South Korea.
J Comp Pathol. 2005 Feb-Apr;132(2-3):139-44. doi: 10.1016/j.jcpa.2004.09.003.
Six pregnant sows were inoculated intranasally at 3 weeks before the expected farrowing date with porcine circovirus 2 (PCV2). The PCV2-inoculated sows showed abortion and premature farrowing, whereas two uninfected negative control sows remained clinically healthy and farrowed normally. PCV2 antigen and DNA were detected by immunohistochemistry and in-situ hybridization, respectively, in lymph node, spleen, thymus, lung, tonsil and liver from both stillborn and liveborn piglets. Simultaneous detection of viral protein and DNA provided molecular evidence of PCV2 infection and replication. The experiment suggested that PCV2 is capable of crossing the placenta, replicating primarily in lymphoid tissues, and inducing reproductive failure.
在预计分娩日期前3周，对6头怀孕母猪进行鼻内接种猪圆环病毒2型（PCV2）。接种PCV2的母猪出现流产和早产，而2头未感染的阴性对照母猪保持临床健康并正常分娩。通过免疫组织化学和原位杂交分别在死产和活产仔猪的淋巴结、脾脏、胸腺、肺、扁桃体和肝脏中检测到PCV2抗原和DNA。病毒蛋白和DNA的同时检测提供了PCV2感染和复制的分子证据。该实验表明，PCV2能够穿过胎盘，主要在淋巴组织中复制，并导致繁殖失败。
