Lodge-Ivey Shanna L, May Tammy, Petersen Mark K, Strickland James R
Animal and Range Science, New Mexico State University, Las Cruces, New Mexico 88001, USA.
J Agric Food Chem. 2004 Nov 17;52(23):6875-8. doi: 10.1021/jf049736w.
There is no reported method for the quantification of methylglyoxal in ruminal fluid. The method reported here is based on the conversion of methylglyoxal to 6-methylpterin, followed by quantification of the resulting pteridinic compound by fluormetric detection using liquid chromatography. Ruminal fluid was collected and preserved with 1 M HCl at -20 degrees C. Cation exchange prior to derivatization was used to eliminate possible interfering peaks. The detection limit of 0.125 microg/mL was calculated. The recoveries were >80%, and the coefficients of variation were <15%. This method has proven to be rugged and accurate for the detection of methylglyoxal concentration in ruminal fluid collected from cows fed diets deficient in degradable intake protein as a marker. Methylglyoxal is produced by ruminal bacteria in response to low nitrogen levels in the rumen. The ruminal methylglyoxal concentration has the potential to be a useful marker to assess ruminal nitrogen status to aid in more accurate diet formulation.
目前尚无关于瘤胃液中甲基乙二醛定量分析的报道方法。本文报道的方法基于甲基乙二醛转化为6-甲基蝶呤，然后通过液相色谱荧光检测对生成的蝶啶类化合物进行定量。收集瘤胃液并用1 M盐酸在-20℃下保存。衍生化前进行阳离子交换以消除可能的干扰峰。计算出检测限为0.125微克/毫升。回收率>80%，变异系数<15%。已证明该方法对于检测从喂食缺乏可降解摄入蛋白日粮的奶牛采集的瘤胃液中的甲基乙二醛浓度是耐用且准确的。甲基乙二醛是瘤胃细菌在瘤胃中低氮水平下产生的。瘤胃甲基乙二醛浓度有可能成为评估瘤胃氮状态的有用标志物，以帮助更准确地配制日粮。
