Wang Jyh-Jye, Lee Chung-Lin, Pan Tzu-Ming
Department of Industrial Safety & Hygiene, Tajen Institute of Technology, Pingdon, Taiwan, Republic of China.
J Agric Food Chem. 2004 Nov 17;52(23):6977-82. doi: 10.1021/jf049783o.
Monascus purpureus NTU 601 is a strain that produces monacolin K, gamma-aminobutyric acid (GABA), and citrinin under solid culture conditions. Because citrinin is a mycotoxin and possesses nephrotoxic and hepatoxic effects, it has a negative impact on the acceptance of red mold rice by people. In this research, a simple and quick selection method for mutant strains with low citrinin production was designed based on the fact that citrinin possesses antibacterial activity for Bacillus subtilis and will form an inhibition zone around the colony of the Monascus strain. The mutant strain M. purpureus N 301 only produced 0.23 +/- 0.01 ppm citrinin, which was 50% less than that of the parent strain, and the monacolin K production was 481.29 +/- 7.98 ppm and maintained 91% productivity. M. purpureus N 310, the other mutant strain, produced 0.27 +/- 0.01 ppm citrinin, which was 41% less than that of the parent strain, and the monacolin K production was 526.29 +/- 5.54 ppm, which showed no significant changes when compared with the parent strain. The GABA content of the two strains was 5000 ppm, which is similar to that of the parent strain. The results showed that the method could be used to select red mold rice with low citrinin production.
紫红曲霉NTU 601是一种在固体培养条件下能产生莫纳可林K、γ-氨基丁酸（GABA）和桔霉素的菌株。由于桔霉素是一种霉菌毒素，具有肾毒性和肝毒性作用，它对人们接受红曲米有负面影响。在本研究中，基于桔霉素对枯草芽孢杆菌具有抗菌活性且会在紫红曲霉菌落周围形成抑菌圈这一事实，设计了一种简单快速的低桔霉素产生突变菌株筛选方法。突变菌株紫红曲霉N 301仅产生0.23±0.01 ppm桔霉素，比亲本菌株少50%，莫纳可林K产量为481.29±7.98 ppm，生产力维持在91%。另一个突变菌株紫红曲霉N 310产生0.27±0.01 ppm桔霉素，比亲本菌株少41%，莫纳可林K产量为526.29±5.54 ppm，与亲本菌株相比无显著变化。这两个菌株的GABA含量为5000 ppm，与亲本菌株相似。结果表明，该方法可用于筛选低桔霉素产生的红曲米。
