Velu Chinavenmeni S, Munuswamy Natesan
Department of Zoology, Life Sciences Building, University of Madras, Guindy Campus, Chennai 600 025, India.
J Agric Food Chem. 2004 Nov 17;52(23):7033-8. doi: 10.1021/jf0490605.
Fatty acids are the largest component of lipids and have become a useful tool in the determination of live feeds to a variety of cultured species. Bioencapsulation is a technique which allows high-level incorporation of desired components (i.e., fatty acids, vitamins, antibiotics, etc.) in live feeds, which in turn can be supplemented to the consumer organisms. The procedure described in the present study serves as a platform of technology for enriching the Streptocephalus dichotomus. Uptake of two enrichment diets (ALGAMAC2000 and DHA-SELCO) by adult S. dichotomus was investigated. The fatty acid profile supports the hypothesis that the enrichment diet increases the level of essential fatty acids, such as linolic, linolenic, eicosapentenoic, and docosahexaenoic acids. The average content (percent of total fatty acids detected) of the enriched organism by different highly unsaturated fatty acid (HUFA) products were as follows: ALGAMAC2000 showed 14-22% saturated fatty acid (SFA), 17-18% monounsaturated fatty acid (MUFA), 28-41% polyunsaturated fatty acid (PUFA), 23-34% n-3, and 4.9-7.5% n-6, whereas DHA-SELCO showed about 20-23% SFA, 20-26% MUFA, 38% PUFA, 28-31% n-3, and 7.5-10% n-6. Our present investigation proves that both HUFA-rich diets appear to be an appropriate enrichment diet, and further provides an additional rationale for using fairy shrimp as a maturation diet for any cultivable freshwater organism.
脂肪酸是脂质的最大组成部分，已成为确定多种养殖物种活饵料的有用工具。生物包囊技术是一种能使所需成分（如脂肪酸、维生素、抗生素等）在活饵料中高水平掺入的技术，进而可将这些活饵料投喂给消费生物。本研究中描述的程序是用于富集双叉臂尾轮虫的技术平台。研究了成年双叉臂尾轮虫对两种强化饵料（ALGAMAC2000和DHA-SELCO）的摄取情况。脂肪酸谱支持这样的假设，即强化饵料能提高必需脂肪酸的水平，如亚油酸、亚麻酸、二十碳五烯酸和二十二碳六烯酸。不同高度不饱和脂肪酸（HUFA）产品对强化生物的平均含量（占检测到的总脂肪酸的百分比）如下：ALGAMAC2000显示饱和脂肪酸（SFA）为14 - 22%，单不饱和脂肪酸（MUFA）为17 - 18%，多不饱和脂肪酸（PUFA）为28 - 41%，n - 3为23 - 34%，n - 6为4.9 - 7.5%；而DHA-SELCO显示SFA约为20 - 23%，MUFA为20 - 26%，PUFA为38%，n - 3为28 - 31%，n - 6为7.5 - 10%。我们目前的研究证明，两种富含HUFA的饵料似乎都是合适的强化饵料，并进一步为将丰年虾用作任何可养殖淡水生物的成熟饵料提供了额外的理论依据。
