与四氯化碳的作用相比，溴三氯甲烷、氯仿和1,1-二氯乙烯对离体大鼠肝细胞磷脂酶A2激活和细胞损伤的影响

Phospholipase A2 activation and cell injury in isolated rat hepatocytes exposed to bromotrichloromethane, chloroform, and 1,1-dichloroethylene as compared to effects of carbon tetrachloride.

作者信息

Glende E A, Recknagel R O

机构信息

Department of Physiology & Biophysics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4970.

出版信息

Toxicol Appl Pharmacol. 1992 Mar;113(1):159-62. doi: 10.1016/0041-008x(92)90021-j.

DOI:10.1016/0041-008x(92)90021-j

PMID:1553751

Abstract

It was found that the four toxigenic agents, CCl4, CHCl3, CBrCl3, and 1,1-dichloroethylene (vinylidene chloride) all share the property of activating phospholipase A2 (PLA2) of isolated hepatocytes in suspension, as determined over a 60- or 120-min time period. In all cases, PLA2 activation, measured as the appearance of lysophosphatidylethanolamine, preceded the release of lactic dehydrogenase during incubation of the cells at 37 degrees C. It is concluded that for these halogenated hydrocarbons phospholipase A2 activation may be part of the chain of causality leading from initial bioactivation to ultimate cell death.

摘要

研究发现，四种产毒剂，即四氯化碳、氯仿、三溴氯甲烷和1,1 - 二氯乙烯（偏二氯乙烯），在60分钟或120分钟的时间段内测定时，都具有激活悬浮培养的分离肝细胞中磷脂酶A2（PLA2）的特性。在所有情况下，以溶血磷脂酰乙醇胺的出现来衡量的PLA2激活，在37℃培养细胞期间乳酸脱氢酶释放之前就已发生。得出的结论是，对于这些卤代烃，磷脂酶A2激活可能是从初始生物活化到最终细胞死亡的因果链的一部分。