Glende E A, Pushpendran C K
Biochem Pharmacol. 1986 Oct 1;35(19):3301-7. doi: 10.1016/0006-2952(86)90427-2.
Freshly isolated rat hepatocytes were exposed to carbon tetrachloride (CCl4) for periods up to 4 hr. Phospholipase A2 activity of these preparations was determined by measuring either the release of [3H]arachidonic acid from cellular phospholipids prelabeled with [3H]arachidonic acid or by measuring the formation of [14C]lysophosphatidylethanolamine from cellular lipids prelabeled with [14C]ethanolamine. Through the use of hexane-partition extraction and thin-layer chromatographic analysis of hepatocyte lipid extracts it was found that CCl4 stimulated phospholipase A2 activity in a dose- and time-dependent manner. Carbon tetrachloride at concentrations of 0.23 to 1.3 mM produced a 1.4- to 5.3-fold increase in phospholipase activity which was initiated within 30-60 min of incubation at 37 degrees. The role of phospholipase activation as a secondary mechanism of CCl4-induced hepatocyte injury is discussed.
将新鲜分离的大鼠肝细胞暴露于四氯化碳（CCl4）中长达4小时。通过测量从预先用[3H]花生四烯酸标记的细胞磷脂中释放的[3H]花生四烯酸，或通过测量从预先用[14C]乙醇胺标记的细胞脂质中形成的[14C]溶血磷脂酰乙醇胺，来测定这些制剂的磷脂酶A2活性。通过对肝细胞脂质提取物进行己烷分配萃取和薄层色谱分析，发现CCl4以剂量和时间依赖性方式刺激磷脂酶A2活性。浓度为0.23至1.3 mM的四氯化碳使磷脂酶活性增加了1.4至5.3倍，这种增加在37℃孵育30至60分钟内开始。讨论了磷脂酶激活作为CCl4诱导的肝细胞损伤的次要机制的作用。
