Liu Junhong, Zhang Yuanyuan, Qiu Long hui, Yang Fengke, Ye Lin, Xia Yamu
Qingdao University of Science and Technology, Box 70, Zhengzhou Road 53, Qingdao, Shandong, China.
J Ind Microbiol Biotechnol. 2004 Dec;31(11):495-9. doi: 10.1007/s10295-004-0174-1. Epub 2004 Nov 9.
A biotransformation process was developed for the production of (S)-ketoprofen by enantioselective hydrolysis of racemic ketoprofen ester using the mutant Trichosporon laibacchii strain CBS 5791. A satisfactory result was obtained, in which the E was 82.5, with an ee of 0.94 and a conversion of 0.47 under the optimum hydrolysis conditions [E is enantiomeric ratio, E=ln[1-X(1+ee)]/ln[1-X(1-ee)]; ee is enantiomeric excess, ee=(CS-CR)/(CS+CR): temperature of hydrolysis was 23 degrees C]. The medium used in biotransformation was a mixture of growth broth and biotransformation broth at a ratio of 1:9, the concentration of Tween 80 was 15 g/l, the time of hydrolysis, 72 h. These results are promising for further scale-up. Tween 80 significantly improved lipase enantioselectivity and activity at the optimum concentration.
通过使用突变型白地霉CBS 5791菌株对消旋酮洛芬酯进行对映选择性水解，开发了一种生产（S）-酮洛芬的生物转化工艺。获得了令人满意的结果，在最佳水解条件下，对映体比例（E）为82.5，对映体过量（ee）为0.94，转化率为0.47 [E为对映体比例，E = ln[1 - X(1 + ee)] / ln[1 - X(1 - ee)]；ee为对映体过量，ee = (CS - CR) / (CS + CR)：水解温度为23℃]。生物转化中使用的培养基是生长肉汤和生物转化肉汤按1:9比例混合而成，吐温80浓度为15 g/l，水解时间为72小时。这些结果对于进一步扩大规模很有前景。在最佳浓度下，吐温80显著提高了脂肪酶的对映选择性和活性。
