Lee Kwang-Woo, Shin Gab-Sang, Bae Hyun-Ae, Shin Hyun-Dong, Lee Yong-Hyun
Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, S. Korea.
Biotechnol Lett. 2004 Nov;26(21):1639-42. doi: 10.1007/s10529-004-3512-5.
A new Acinetobacter sp. ES-1, grown on triolein, tryptone and Triton X-100, excreted a lipase that hydrolyzed 10 mM (R,S)-ketoprofen ethyl ester into (S)-ketoprofen. The crude lipase had an activity of 10 U ml(-1) and, at 30 degrees C and pH 7 over 48 h, gave a conversion yield of 35% with an enantiomeric excess for the product 96%.
一种新的不动杆菌属菌株ES-1，在三油酸甘油酯、胰蛋白胨和吐温X-100上生长，分泌出一种脂肪酶，该脂肪酶可将10 mM（R,S）-酮洛芬乙酯水解为（S）-酮洛芬。粗脂肪酶的活性为10 U ml⁻¹，在30℃和pH 7条件下反应48小时，产物的转化率为35%，对映体过量值为96%。
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