Zhao Yan, Yang Li-Fang, Ye Mao, Gu Huan-Hua, Cao Ya
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, Hunan, PR China.
Prev Med. 2004 Dec;39(6):1172-9. doi: 10.1016/j.ypmed.2004.04.042.
To elucidate the apoptosis induction of Epigallocatechin-3-gallate (EGCG) on nasopharyngeal carcinoma (NPC) cells via mitochondrial signal transduction pathway regulated by EB-virus-encoded latent membrane protein 1 (LMP1).
The survival rates of pTet-on-LMP1 HNE2 cells after the EGCG treatment were determined by MTT assay. Induction of apoptosis in pTet-on-LMP1 HNE2 cells after the EGCG treatment was analyzed by agarose gel electrophoresis. The activity of caspase-9 was determined by ApoAlert Caspase-9 Fluorescent Assay kit after the EGCG treatment. The protein expressions of cytochrome c and Bcl-2 were analyzed by Western blotting after the EGCG treatment.
EGCG inhibited the survival rates of pTet-on-LMP1 HNE2 cells and induced apoptosis of pTet-on-LMP1 HNE2 cells. EGCG raised the activities of caspase-9, enhanced the releasing of cytochrome c from the mitochondria, and suppressed the protein expression of Bcl-2. These are the key targets on the mitochondrial signal transduction pathway in apoptosis.
EGCG inhibited the survival rate of NPC cells and induced apoptosis of NPC cells via the mitochondrial signal transduction pathway. This study suggests that the interference effect of EGCG on targets of the mitochondrial signal transduction pathway plays an important role in the anticancer function.
阐明表没食子儿茶素-3-没食子酸酯（EGCG）通过由EB病毒编码的潜伏膜蛋白1（LMP1）调控的线粒体信号转导途径对鼻咽癌（NPC）细胞凋亡的诱导作用。
采用MTT法测定EGCG处理后pTet-on-LMP1 HNE2细胞的存活率。通过琼脂糖凝胶电泳分析EGCG处理后pTet-on-LMP1 HNE2细胞凋亡的诱导情况。采用ApoAlert Caspase-9荧光检测试剂盒测定EGCG处理后caspase-9的活性。通过蛋白质免疫印迹法分析EGCG处理后细胞色素c和Bcl-2的蛋白表达。
EGCG抑制pTet-on-LMP1 HNE2细胞的存活率并诱导其凋亡。EGCG提高了caspase-9的活性，增强了细胞色素c从线粒体的释放，并抑制了Bcl-2的蛋白表达。这些是凋亡中线粒体信号转导途径的关键靶点。
EGCG通过线粒体信号转导途径抑制NPC细胞的存活率并诱导其凋亡。本研究表明EGCG对线粒体信号转导途径靶点的干扰作用在其抗癌功能中起重要作用。
