维生素E包被的透析器膜可下调单核细胞黏附分子和共刺激分子的表达。
Vitamin E-coated dialyzer membranes downregulate expression of monocyte adhesion and co-stimulatory molecules.
作者信息
Betjes Michiel G H, Hoekstra Franciska M E, Klepper M, Postma Saskia M, Vaessen Leonard M B
机构信息
Erasmus Medical Center, Dijkzigt Rotterdam, Department of Internal Medicine, Division of Nephrology, Rotterdam, The Netherlands.
出版信息
Blood Purif. 2004;22(6):510-7. doi: 10.1159/000082042. Epub 2004 Nov 10.
BACKGROUND
In patients on chronic hemodialysis leukocyte activation has been related to the impaired function of the immune system. In this study we investigated if the vitamin E-coated dialyzer membrane could reduce monocyte activation thereby improving cellular immunity.
METHODS
This hypothesis was tested in a prospective crossover trial in which 14 stable hemodialysis patients were switched from the baseline hemophane dialyzer to a vitamin E-coated and thereafter a polysulphone dialyzer membrane or vice versa.
RESULTS
Monocyte MHC class I, CD54 and ICAM-1 expression was significantly downregulated when a vitamin E-coated or polysulphone dialyzer was used. The use of a vitamin E membrane specifically decreased monocyte CD40 and CD86 expression. Lectin induced T cell proliferation increased with the use of the vitamin E-coated membrane as compared to polysulphone and hemophane dialyzers.
CONCLUSION
Vitamin E-coated dialyzers induced a less-activated phenotype of monocytes and may improve cellular immunity.
背景
在慢性血液透析患者中，白细胞激活与免疫系统功能受损有关。在本研究中，我们调查了维生素E包被的透析器膜是否能减少单核细胞激活，从而改善细胞免疫。
方法
在一项前瞻性交叉试验中检验了这一假设，14例稳定的血液透析患者从基线血仿膜透析器转换为维生素E包被的透析器，之后再转换为聚砜膜透析器，反之亦然。
结果
使用维生素E包被的或聚砜膜透析器时，单核细胞MHC I类分子、CD54和细胞间黏附分子-1（ICAM-1）的表达显著下调。使用维生素E膜可特异性降低单核细胞CD40和CD86的表达。与聚砜膜和血仿膜透析器相比，使用维生素E包被的膜时，凝集素诱导的T细胞增殖增加。
结论
维生素E包被的透析器可诱导单核细胞呈现激活程度较低的表型，并可能改善细胞免疫。
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