Akiyama Shinichiro, Inagaki Masahiro, Tsuji Mayumi, Gotoh Hiromichi, Gotoh Tomomi, Washio Kazunori, Gotoh Yoshikazu, Oguchi Katsuji
Department of Pharmacology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Am J Nephrol. 2005 Sep-Oct;25(5):500-6. doi: 10.1159/000088172. Epub 2005 Sep 6.
We reported earlier that production of Cu/Zn-superoxide dismutase (SOD) increases markedly in hemodialysis patients but not in non-dialyzed chronic renal failure (CRF) patients. In this study, we compared the antioxidant effects of oral vitamin E supplementation (VE-PO) and vitamin E coating of a dialyzer (VE-BMD) by measuring increased Cu/Zn-SOD in hemodialysis patients.
31 hemodialysis patients were divided into two groups: 16 hemodialysis patients underwent usual dialysis with vitamin E supplementation 600 mg/day while 15 others were dialyzed using vitamin E-coated membrane for 6 months. Total plasma SOD activity was determined by NBT method, plasma Cu/Zn-SOD contents by ELISA and Cu/Zn-SOD mRNA in leukocytes by RT-PCR.
VE-PO and VE-BMD showed almost comparable effects on Cu/Zn-SOD contents and its mRNA levels in hemodialysis patients. VE-PO resulted in a progressive decrease of Cu/Zn-SOD content (p < 0.001). A comparable progressive decrease was observed also in VE-BMD (p < 0.0001). Both VE-PO and VE-BMD resulted in a progressive decrease of Cu/Zn-SOD mRNA (p < 0.01), which reached the level of non-dialyzed CRF patients.
我们之前报道过，血液透析患者体内铜锌超氧化物歧化酶（SOD）的生成显著增加，但未透析的慢性肾衰竭（CRF）患者则不然。在本研究中，我们通过测量血液透析患者体内铜锌超氧化物歧化酶的增加量，比较了口服维生素E补充剂（VE-PO）和透析器维生素E涂层（VE-BMD）的抗氧化作用。
31例血液透析患者被分为两组：16例血液透析患者接受常规透析，并每天补充600毫克维生素E，而另外15例患者使用维生素E涂层膜进行透析6个月。通过NBT法测定血浆总SOD活性，通过ELISA法测定血浆铜锌超氧化物歧化酶含量，通过RT-PCR法测定白细胞中的铜锌超氧化物歧化酶mRNA。
VE-PO和VE-BMD对血液透析患者铜锌超氧化物歧化酶含量及其mRNA水平的影响几乎相当。VE-PO导致铜锌超氧化物歧化酶含量逐渐降低（p < 0.001）。在VE-BMD组也观察到类似的逐渐降低（p < 0.0001）。VE-PO和VE-BMD均导致铜锌超氧化物歧化酶mRNA逐渐降低（p < 0.01），达到未透析CRF患者的水平。
