Fujishima Naohito, Hirokawa Makoto, Aiba Namiko, Ichikawa Yoshikazu, Fujishima Masumi, Komatsuda Atsushi, Suzuki Yoshiko, Kawabata Yoshinari, Miura Ikuo, Sawada Ken-ichi
Department of Internal Medicine III, Akita University School of Medicine, Akita, Japan.
Int J Hematol. 2004 Oct;80(3):239-45. doi: 10.1532/ijh97.04053.
We compared the expression profiles of highly purified human CD34+ cells and erythroid progenitor cells by micro-serial analysis of gene expression (microSAGE). Human CD34+ cells were purified from granulocyte colony-stimulating factor-mobilized blood stem cells, and erythroid progenitors were obtained by cultivating these cells in the presence of stem cell factor, interleukin 3, and erythropoietin. Our 10,202 SAGE tags allowed us to identify 1354 different transcripts appearing more than once. Erythroid progenitor cells showed increased expression of LRBA, EEF1A1, HSPCA, PILRB, RANBP1, NACA, and SMURF. Overexpression of HSPCA was confirmed by real-time polymerase chain reaction analysis. MicroSAGE revealed an unexpected preferential expression of several genes in erythroid progenitor cells in addition to the known functional genes, including hemoglobins. Our results provide reference data for future studies of gene expression in various hematopoietic disorders, including myelodysplastic syndrome and leukemia.
我们通过基因表达微序列分析（microSAGE）比较了高度纯化的人类CD34+细胞和红系祖细胞的表达谱。人类CD34+细胞从粒细胞集落刺激因子动员的血液干细胞中纯化得到，红系祖细胞则通过在干细胞因子、白细胞介素3和促红细胞生成素存在的条件下培养这些细胞获得。我们的10202个SAGE标签使我们能够鉴定出1354种出现不止一次的不同转录本。红系祖细胞显示出LRBA、EEF1A1、HSPCA、PILRB、RANBP1、NACA和SMURF的表达增加。通过实时聚合酶链反应分析证实了HSPCA的过表达。MicroSAGE揭示了除已知功能基因（包括血红蛋白）外，红系祖细胞中几种基因意外的优先表达。我们的结果为未来研究包括骨髓增生异常综合征和白血病在内的各种造血系统疾病中的基因表达提供了参考数据。
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