Vila-Porcile E, Picart R, Vigny M, Tixier-Vidal A, Tougard C
Groupe de Neuroendocrinologie Cellulaire et Moléculaire, Collège de France, Paris.
Anat Rec. 1992 Apr;232(4):482-92. doi: 10.1002/ar.1092320405.
The distribution of three components of basement membranes (BM): heparan-sulfate proteoglycan (HSPG), entactin (ENT), and type IV collagen (Coll. IV), was studied in the adult rat anterior pituitary and compared to the distribution of laminin (LAM) described in an earlier report (Vila-Porcile et al., 1987, J. Histochem. Cytochem., 35, 287). Several immunocytochemical methods were applied at both light and electron microscope levels. The three components were detected in all the pituitary BM and in endothelial and perivascular connective cells, as previously observed for LAM. In contrast to the prominent labeling previously observed for LAM within epithelial cells, however, only a faint immunoreaction could be detected for the other components, with nonetheless a discrete signal for Coll. IV. These findings indicate that 1) the four studied components are present in all the BM of the rat anterior pituitary; 2) pituitary endocrine cells contain LAM, and to a lesser extent Coll. IV, and thus could participate to the elaboration of the BM; and 3) the presence of the four components in non-epithelial cells also suggests their cooperation in BM building. The questions of the turnover and intracellular pathways of each of these components were addressed but remain unanswered.
研究了成年大鼠垂体前叶基底膜（BM）的三种成分：硫酸乙酰肝素蛋白聚糖（HSPG）、巢蛋白（ENT）和IV型胶原（Coll.IV）的分布，并与先前报告（Vila-Porcile等人，1987年，《组织化学与细胞化学杂志》，35卷，287页）中描述的层粘连蛋白（LAM）的分布进行了比较。在光学显微镜和电子显微镜水平上应用了几种免疫细胞化学方法。正如先前对LAM的观察结果一样，在所有垂体基底膜以及内皮细胞和血管周围结缔组织细胞中均检测到这三种成分。然而，与先前在上皮细胞中观察到的LAM的显著标记不同，对于其他成分仅能检测到微弱的免疫反应，尽管Coll.IV有离散信号。这些发现表明：1）所研究的四种成分存在于大鼠垂体前叶的所有基底膜中；2）垂体内分泌细胞含有LAM，在较小程度上含有Coll.IV，因此可能参与基底膜的形成；3）非上皮细胞中四种成分的存在也表明它们在基底膜构建中相互协作。对这些成分各自的更新和细胞内途径问题进行了探讨，但仍未得到解答。
