评估API ID 32C和VITEK-2系统对都柏林念珠菌的鉴定能力。
Evaluation of API ID 32C and VITEK-2 to identify Candida dubliniensis.
作者信息
Cárdenes-Perera Carmen-Delia, Torres-Lana Alvaro, Alonso-Vargas Rocío, Moragues-Tosantas María-Dolores, Pontón-San Emeterio José, Quindós-Andrés Guillermo, Arévalo-Morales María-Pilar
机构信息
Area de Medicina Preventiva y Salud Pública, Facultad de Medicina, Universidad de La Laguna, La Laguna, Spain.
出版信息
Diagn Microbiol Infect Dis. 2004 Nov;50(3):219-21. doi: 10.1016/j.diagmicrobio.2004.06.010.
We have compared two diagnostic systems, API-ID32C (bioMerieux) and VITEK-2ID-YST (bioMerieux), in their ability to diagnose 50 clinical isolates of Candida dubliniensis. API identified 48 isolates and VITEK-2 identified 33 of the 50 isolates. Lactic acid assimilation showed highly dispersed results with API, being of special importance, since this test is one of the four that makes it possible to differentiate C. dubliniensis from C. albicans. The detection of one enzyme, Phosphate-4MU, was always positive with VITEK-2 when it should have been negative. Overall, API ID 32C obtained better results than VITEK-2. However, the latter is simpler to use and has a greater database.
我们比较了两种诊断系统，即API-ID32C（生物梅里埃公司）和VITEK-2 ID-YST（生物梅里埃公司）对50株都柏林念珠菌临床分离株的诊断能力。API系统鉴定出48株，VITEK-2系统在50株中鉴定出33株。利用API系统进行乳酸同化试验的结果差异很大，这一点非常重要，因为该试验是区分都柏林念珠菌和白色念珠菌的四项试验之一。有一种酶（磷酸-4-甲基伞形酮酶）的检测，VITEK-2系统检测结果本应呈阴性时却总是呈阳性。总体而言，API ID 32C系统比VITEK-2系统获得了更好的结果。然而，后者使用起来更简便，且拥有更庞大的数据库。
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