Division of Oncology, Department of Medicine, Charité Universitätsmedizin Berlin, Charité Campus Mitte, Berlin, Germany.
J Microbiol Immunol Infect. 2010 Apr;43(2):147-54. doi: 10.1016/S1684-1182(10)60023-0.
BACKGROUND/PURPOSE: Mixed infections caused by different Candida species are the rule rather than the exception. The discrimination between the two closely related species Candida albicans and Candida dubliniensis is not trivial. Therefore, there is a need for fast, reliable, and inexpensive methods with high specificity for the identification and differentiation of these two Candida species, which are frequently detected in the oral cavities of patients with a human immunodeficiency virus infection.
We applied several phenotypic identification methods (growth on Rice-agar, Bird-seed agar, CHROMagar Candida, API ID 32C; growth at 42 degrees C and 45 degrees C) and compared them with genotyping by arbitrarily primed-polymerase chain reaction.
A sensitivity of 44% for the identification of C. dubliniensis was achieved for growth on Rice-agar, 97% for discrimination on Bird-seed agar, 95% with the assimilation profile index API ID 32C, and 97% when grown at 45 degrees C. We found two API codes not described for C. dubliniensis so far. Additionally, 88% of our C. dubliniensis isolates assimilated palatinose, in contrast to the 1% described in the API reference manual.
According to our results, cultivation of Candida isolates on Bird-seed agar after screening on CHROMagar Candida is a very sensitive, simple, and cost-effective method for discriminating C. dubliniensis from C. albicans in routine practice.
背景/目的：不同念珠菌物种引起的混合感染是常见情况，而非例外。区分两种密切相关的物种，白色念珠菌和都柏林念珠菌，并非易事。因此，需要快速、可靠且经济实惠的方法，具有高特异性，用于鉴定和区分这两种经常在人类免疫缺陷病毒感染患者口腔中检测到的念珠菌。
我们应用了几种表型鉴定方法（在大米琼脂、鸟籽琼脂、CHROMagar Candida、API ID 32C 上生长；在 42 度和 45 度下生长），并将其与任意引物聚合酶链反应（AP-PCR）的基因分型进行了比较。
在大米琼脂上生长的方法对鉴定都柏林念珠菌的敏感性为 44%，鸟籽琼脂上的区分率为 97%，API ID 32C 的同化谱指数为 95%，在 45 度下生长的识别率为 97%。我们发现了两种迄今为止尚未在 API 参考手册中描述的代码。此外，我们 88%的都柏林念珠菌分离株能够同化棉子糖，而 API 参考手册中描述的为 1%。
根据我们的结果，在 CHROMagar Candida 筛选后，将念珠菌分离株在鸟籽琼脂上培养，是一种在常规实践中区分白色念珠菌和都柏林念珠菌的非常敏感、简单且具有成本效益的方法。
