植物中无标记的位点特异性基因整合

Marker-free site-specific gene integration in plants.

作者信息

Srivastava Vibha, Ow David W

机构信息

Crop, Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR 72701, USA.

出版信息

Trends Biotechnol. 2004 Dec;22(12):627-9. doi: 10.1016/j.tibtech.2004.10.002.

DOI:10.1016/j.tibtech.2004.10.002

PMID:15542152

Abstract

For nearly 15 years, the use of site-specific recombination systems in plants has focused on the deletion or integration of DNA. Each of these applications offers practical solutions to two problems in biotechnology: the presence of unneeded DNA in the transgene locus and variation in the locus structure among independent transgenic lines. Given that each of these separate applications is becoming more practical for commercial use, it is time to consider combining them into an integrated technology. Here we propose a strategy for a "combined step" method that makes use of two site-specific recombination systems: one for integrating the DNA and a second for removing sequences that are not needed after DNA transfer. This strategy is based on published data and exemplifies the use of the Cre-lox, FLP-FRT and R-RS inducible systems.

摘要

近15年来，植物中位点特异性重组系统的应用主要集中在DNA的缺失或整合上。这些应用中的每一种都为生物技术中的两个问题提供了切实可行的解决方案：转基因位点中存在不需要的DNA以及独立转基因品系间位点结构的变异。鉴于这些单独的应用对于商业用途而言正变得越来越实用，现在是时候考虑将它们整合为一项综合技术了。在此，我们提出一种 “组合步骤” 方法的策略，该方法利用两种位点特异性重组系统：一种用于整合DNA，另一种用于去除DNA转移后不需要的序列。该策略基于已发表的数据，并举例说明了Cre-lox、FLP-FRT和R-RS诱导系统的应用。